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Patricia DULOR Anthony BOUTER Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Yann FERRAND Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU

Corinne Loutelier-Bourhis, 16/12/2015, 16h

  The seminar will take place, the 16 december 2015 at 16h, at the  ENSCBP, Building B, room 1, allée Geoffroy Saint-Hilaire, Pessac.

 

 

Ion Mobility Spectrometry-Mass Spectrometry Contributions and limits in chemical and biochemical applications

 

Corinne Loutelier-Bourhis

Associate Professor, University of Rouen.

 

Abstract

 

 

  Since few years, the coupling of mass spectrometry to ion mobility spectrometry (IMS-MS) proved to be particularly useful for the analyses of complex mixtures, the differentiation of isomers or isobars and the study of biomolecule conformations, through the separation of ions according to their charge, size and shape (IMS dimension) and m/z ratio (MS dimension).

 

  This two-dimension separation technique allows an increase of peak capacity and of structural information. Indeed, structural characteristics concerning the conformation of compounds can be attained through IM-MS measurements of the collision cross sections (CCS) which can be correlated with calculated CCS values using both molecular dynamics or DFT calculations and MOBCAL methods.

 

  Here, the contribution of IMS-MS is presented through some examples of isomers separation, polymer and biomolecule analyses showing that this approach can advantageously complement the conventional mass spectrometric methodologies. The limits of this recent technique are also depicted.

 

 

 

 

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