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Sophie Zinn-Justin, 14 April 2017, ... Christina Sizun, 18 May 2017, 4 pm,... Cécile Feuillie, 16 February 2017,... Félix M. Goñi, 8 december 2016, 4... Félix M. Goñi, 17 november 2016, ... Carl Creutz, 20 october 2016, 4 pm,... Diego Romero, 30 september 2016, 11... A. Ciaccafava, 8 september 2016, 14... A. Ramamoorthy, 16 June 2016, 16h,... Alexandre de Brevern, 2 june 2016, ... Isabelle Landrieu, 26 May, 16h, ENS... Frances Sepavoric, 12 May, 16h, ENS... Thomas Pradeu, 7 April 2016, 9h, EN... Françoise Argoul, 3 march 2016, 1... Corinne Loutelier-Bourhis, 16/12/20... Aristotelis XENAKIS, 3 december 201... Fabian Kiessling, 26 november 2015,... Michaël Molinari, 20 november 2015... Dipankar Das Sarma, 28 October 2015... Olivier Donard, 22 october 2015, 16... E. Morvan & A.Grelard, 17/12/2015, ... Christophe Cullin, 10 september 201... Brigitte Lindet, 18 June 2015, 16h,... Marion Decossas, 4 June 2015, 16h, ... Pascale Schellenberger, 21 Mai 2015... F. Leal-Calderon, 7 May 2015, 16h, ... Ibrahim Abdulhalim, 9 April 2015, 1... Manon Carré, 26 March 2015, 14h, ... C. Bure & JM Schmitter, 19 March 20... T. Ogata & H. Ihara, 17 March 2015,... Banafshe Larijani, 12 March 2015, 1...
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Patricia DULOR Alain BRISSON Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Ivan HUC Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU

Sophie Zinn-Justin, 14 April 2017, 11 am, IECB

The seminar will take place, the 14th of April 2017, at 11 am, in the IECB amphitheatre, 2 rue Robert Escarpit Pessac.

 

Describing the tailed bacteriophage virion from a combination of NMR and EM data

 

Dr. Sophie Zinn-Justin

CEA, Saclay

 

Abstract

 

Most known bacteriophages are constituted by a capsid (or head) containing their genome and a tail that serve for bacterial target recognition and genome delivery into the infected host cytoplasm. We analyzed the phage protein divergent sequences in order to identify genes coding for the capsid, head-to-tail connection and tail of the phage viral particle (Lopes et al., 2014). We thus classified phages as a function of their head-to-tail connection architecture.We focused on phage SPP1, which is representative of the largest phage class, in order to describe the architecture of its viral particle.

 

We solved the 3D solution structures of four SPP1 proteins before assembly into the viral particle; these structures are characterized by the presence of large flexible loops (Lhuillier et al., 2009; Chagot et al., 2012). We then docked these structures into the EM maps of the head-to-tail connection of the SPP1 virion in two functional states, i.e. before and after DNA ejection (Tavares et al., 2012; Chaban et al., 2015). Loops involved in capsid closure were identified and this was validated by observing disulfide cross-linking between the loops rearranged as b-strands in the assembled particle (Lhuillier et al., 2009; Chaban et al., 2015). In long-tail phages as SPP1, the tail tube results from the assembly of the Major Tail Protein (MTP) gp17.1. We showed that monomeric gp17.1 is partially folded in solution and self-assembles to form native-like fibers. We followed, using solution-state NMR, FTIR, solid-state NMR and EM, the structural changes experienced by gp17.1 during fiber formation, and proposed a 3D model for the gp17.1 fiber (Langlois et al., 2015). Finally, we validated our model by designing a gp17.1 variant with impaired assembly capacities in vitro and in the bacteria. Similarities between virion architectures within tailed bacteriophages will be discussed.

 

short CV

 

Since 10-15 years, Dr Sophie Zinn-Justin heads a group interested in the description of protein complex assembly either in the viral particle of tailed bacteriophages or in the nuclear envelope of human cells. Her team belongs to the Laboratory of Structural biology and Radiobiology” of the Institute of Integrated Biology of the Cell (I2BC, Université Paris Saclay). Combining different approaches in Structural Biology with an emphasis on solution NMR, she focuses on the understanding of the assembly mechanisms and functions of bacteriophage and human nuclear envelope proteins.

 

Contact: Birgit Habenstein, b.habenstein@iecb.u-bordeaux.fr, Antoine Loquet, a.loquet@iecb.u-bordeaux.fr

 

 

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