Institut de Chimie & Biologie des Membranes & des Nano-objets • Bordeaux

LIA France-Japan GEM Réseau RMN Aquitain Master AC2QMAPS IPEP master UREKA UMT FOLIES INDIA
Offres d'Emploi Plateau Technique Conseil d'Unité Animation Scientifique WEB Editorial Committee Hygiène & Sécurité Communication Administration Directoire Scientifique Direction
Emmanuelle Thinon, 28 June 2017, 11 am, IECB Caroline Tokarski, 15 June 2017, 4pm, ENSCBP Christian Salesse, 8 June 2017, 4pm, ENSCBP Patrice Rey, 01 June 2017, 2h30 pm, ENSCBP Christina Sizun, 18 May 2017, 4 pm, ENSCBP Marisela Velez, 5 May 2017, 11am, IECB Iqbal Choudhary, 27 April 2017, 4 pm, ENSCBP Vincent Aucagne, 21 April 2017, 11 am, IECB Sophie Zinn-Justin, 14 April 2017, 11 am, IECB Cécile Feuillie, 16 February 2017, 4 PM, ENSCBP Félix M. Goñi, 8 december 2016, 4 pm, ENSCBP Félix M. Goñi, 17 november 2016, 4 pm, ENSCBP Carl Creutz, 20 october 2016, 4 pm, ENSCBP Diego Romero, 30 september 2016, 11 am, IECB A. Ciaccafava, 8 september 2016, 14h, ENSCBP A. Ramamoorthy, 16 June 2016, 16h, ENSCBP Alexandre de Brevern, 2 june 2016, ENSCBP Isabelle Landrieu, 26 May, 16h, ENSCBP Frances Sepavoric, 12 May, 16h, ENSCBP Thomas Pradeu, 7 April 2016, 9h, ENSCBP Françoise Argoul, 3 march 2016, 16h, ENSCBP Corinne Loutelier-Bourhis, 16/12/2015, 16h Aristotelis XENAKIS, 3 december 2015, 16h Fabian Kiessling, 26 november 2015, 11h Michaël Molinari, 20 november 2015, 11h Dipankar Das Sarma, 28 October 2015, 14h. Olivier Donard, 22 october 2015, 16h, ENSCBP E. Morvan & A.Grelard, 17/12/2015, 16h, ENSCBP Christophe Cullin, 10 september 2015, 14 h Brigitte Lindet, 18 June 2015, 16h, ENSCBP(B) Marion Decossas, 4 June 2015, 16h, ENSCBP(B) Pascale Schellenberger, 21 Mai 2015, 16h F. Leal-Calderon, 7 May 2015, 16h, ENSCBP(B) Ibrahim Abdulhalim, 9 April 2015, 14h, ENSCBP Manon Carré, 26 March 2015, 14h, ENSCBP(B) C. Bure & JM Schmitter, 19 March 2015, 16h T. Ogata & H. Ihara, 17 March 2015, 11h, IECB Banafshe Larijani, 12 March 2015, 16h, ENSCBP
Événements Nouveau
Partenariat Congés Formation Admin CBMN Conseil Institut/Scientifique
Affiliations Les sites CBMN
LIA France-Japan GEM Réseau RMN Aquitain Master AC2QMAPS IPEP master UREKA UMT FOLIES INDIA
Offres d'Emploi Plateau Technique Conseil d'Unité Animation Scientifique WEB Editorial Committee Hygiène & Sécurité Communication Administration Directoire Scientifique Direction
Chimie Biophysique Chimie Biomimétique et Thérapeuti... Biologie et Biotechnologie Administration
Emmanuelle Thinon, 28 June 2017, 11... Caroline Tokarski, 15 June 2017, 4p... Christian Salesse, 8 June 2017, 4pm... Patrice Rey, 01 June 2017, 2h30 pm,... Christina Sizun, 18 May 2017, 4 pm,... Marisela Velez, 5 May 2017, 11am, I... Iqbal Choudhary, 27 April 2017, 4 p... Vincent Aucagne, 21 April 2017, 11 ... Sophie Zinn-Justin, 14 April 2017, ... Cécile Feuillie, 16 February 2017,... Félix M. Goñi, 8 december 2016, 4... Félix M. Goñi, 17 november 2016, ... Carl Creutz, 20 october 2016, 4 pm,... Diego Romero, 30 september 2016, 11... A. Ciaccafava, 8 september 2016, 14... A. Ramamoorthy, 16 June 2016, 16h,... Alexandre de Brevern, 2 june 2016, ... Isabelle Landrieu, 26 May, 16h, ENS... Frances Sepavoric, 12 May, 16h, ENS... Thomas Pradeu, 7 April 2016, 9h, EN... Françoise Argoul, 3 march 2016, 1... Corinne Loutelier-Bourhis, 16/12/20... Aristotelis XENAKIS, 3 december 201... Fabian Kiessling, 26 november 2015,... Michaël Molinari, 20 november 2015... Dipankar Das Sarma, 28 October 2015... Olivier Donard, 22 october 2015, 16... E. Morvan & A.Grelard, 17/12/2015, ... Christophe Cullin, 10 september 201... Brigitte Lindet, 18 June 2015, 16h,... Marion Decossas, 4 June 2015, 16h, ... Pascale Schellenberger, 21 Mai 2015... F. Leal-Calderon, 7 May 2015, 16h, ... Ibrahim Abdulhalim, 9 April 2015, 1... Manon Carré, 26 March 2015, 14h, ... C. Bure & JM Schmitter, 19 March 20... T. Ogata & H. Ihara, 17 March 2015,... Banafshe Larijani, 12 March 2015, 1...
Événements Nouveau
Patricia DULOR Alain BRISSON Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Ivan HUC Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU

Marisela Velez, 5 May 2017, 11am, IECB

The seminar will take place, the 5th of May 2017, at 11 am, in the IECB amphitheatre, 2 rue Robert Escarpit Pessac

 

What assemblies of bacterial cytoskeletal protein FtsZ filaments on surfaces observed in vitro suggest about the generation of a contractile force in vivo

 

Dr. Marisela VELEZ

Institute of Catalysis and Petrochemistry CSIC

 

Abstract

 

FtsZ is a cytoskeletal protein that participates in the formation, on the inner side of the cytoplasmic bacterial membrane, of the “septal ring”, a protein complex responsible for cell division [1]. We have used supported lipid bilayers and controlled orientation of FtsZ monomers in vitro to study their GTP-dependent self-assembling on surfaces with atomic force microscopy in solution, providing single molecule information of the dynamic structure of filaments and their aggregates [2-4]. A theoretical description of individual filaments that incorporates information from molecular dynamic simulations has identified that filament curvature, monomer twist, surface attachment and lateral interactions between monomers are enough to describe, using Monte Carlo simulations, some of the structures that we observe experimentally.

 

The implication of this work is that monomer flexibility and surface attachment, additionally to filament curvature, could be important in determining the polymorphism and dynamic behavior of filament aggregates. Furthermore, we suggest a new mechanism for force generation in which the orientation and type of monomer attachment to the surface could play an important role in modulating the force exerted by filament aggregates on the bacterial membrane during cell division [5]. Ongoing work is directed towards testing the hypothesis that monomer attachment, flexibility and conformation are associated with the shape, rigidity and dynamics of the filaments and that this is relevant for creating tensions on the membrane.

 

  1. Mingorance, J.; Rivas, G.,Vélez; M.,Gómez-Puertas, P.; Vicente, M. “Strong FtsZ is with the force: mechanisms to constrict bacteria” Trends in Microbiology 18, 348-356, (2010)

  2. Mateos-Gil , P.; Paez ,A.; Hörger, I, Rivas, G.; Vicente,M.; Tarazona,P. and Vélez, M. “Depolymerization dynamics of individual filaments of bacterial cytoskeletal protein FtsZ” PNAS 109 8133‐8138. (2012)

  3. González de Prado Salas, P., Encinar,M., Vélez,M. and Tarazona,P. “ FtsZ protein on bilayer membranes: effects of specific lateral bonds “ Soft Matter 9, 6072 (2013)

  4. Encinar, M. ; Kralicek, A.; Martos, A.; Krupka, M.; Alonso, A.; Cid, Sa.; Rico, A.; Jiménez, M.; Vélez, M.“ Polymorphism of FtsZ filaments on lipid surfaces: role of monomer orientation" Langmuir 29, 9436 – 9446 (2013)

  5. González de Prado Salas,P., Hörger,I., Martín-García,F.,J Mendieta,J.; Alvaro,A., Encinar,M., Gómez-Puertas,P., Vélez, M., and Tarazona,P. “Torsion and curvature of FtsZ" Soft Matter 10; 1977- 1986 (2014)

 

Curriculum Vitae

 

BACHELOR IN SCIENCE (B.S.): Biology (biochemistry) Autonomus Universtity of Madrid . June 1982

DOCTOR OF PHILOSOPHY (Ph.D): Biophysics. University of Michigan (USA) August 1989

 

Research Scientist

Instituto de Catálisis y Petroleoquímica (Catalysis and Oil-chemistry Institute) C.S.I.C.

POSTAL ADDRESS: C/ Marie Curie, 2 ; Cantoblanco ; Madrid E-28049

(SPAIN) TEL: +34 91 585 4800; FAX:+34 91 585 4760

marisela.velez@uam.es mvelez@icp.csic.es

http://www.icp.csic.es/personal.php?id=258

 

RESEARCH INTERESTS:

The main research interest of my group is to understand biological processes on surfaces using in vitro reconstituted systems. We address questions regarding the structure and function of macromolecular aggregates formed on lipid surfaces, particularly those relevant for bacterial cell division, using biophysical techniques as AFM, QCM, fluorescence microscopy. We combine the single molecule description of the structure and dynamic behavior of the polymers of bacterial protein FtsZ on different surfaces with theoretical analysis to understand at the molecular level the force generating mechanism of this self-assembling protein.

We also collaborate closely with the bioelectrocatalysis group to nanostructure and characterize gold electrodes

with lipidic membranes to incorporate active membrane redox proteins.

 

Contact: Birgit Habenstein, b.habenstein@iecb.u-bordeaux.fr, Antoine Loquet, a.loquet@iecb.u-bordeaux.fr

 

 

 

 

CBMN Accueil Intro Actualités Séminaires Pubs (WS) Plateformes
Départements Administration Biologie et Biotechnologie Chimie Biomimétique et Thérapeutique Chimie Biophysique
Un problème sur le site? Contactez notre webmaster : webmaster@cbmn.u-bordeaux.fr
Services Direction Directoire Scientifique Administration Communication Hygiène & Sécurité WEB Editorial Committee Animation Scientifique Conseil d'Unité Plateau Technique Offres d'Emploi
EXT INDIA UMT FOLIES UREKA IPEP master Master AC2QMAPS Réseau RMN Aquitain GEM LIA France-Japan
Équipes Patricia DULOR Alain BRISSON Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Ivan HUC Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU
informations Mentions légales Plan d'accès Réalisation : Little Big Studio
Dernière mise à jour : 17/08/2017

CNRS Université de Bordeaux Institut Polytechnique de Bordeaux

CBMN Allée de Geoffroy St Hilaire Bât B14
33600 Pessac
Tél. : +33 (0)5 40 00 68 00
Fax : +33 (0)5 40 00 22 00
Mail : contact@cbmn.u-bordeaux.fr