Institut de Chimie & Biologie des Membranes & des Nano-objets • Bordeaux

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Christian Griesinger, November 24th, 11am, IECB R. Dos santos Morais, 30/11/2017, 4 PM, ENSCBP Takahiro Muraoka, 16/11/2017, 11am, ENSCBP Vladimir Torbeev, 9/11/2017, 2pm, ENSCBP M. D. Smith & T.Constantieux, 3/11/2017, 2h30 pm, IECB James Nowick, 15 september 2017, 11am, IECB Emmanuelle Thinon, 28 June 2017, 11 am, IECB Caroline Tokarski, 15 June 2017, 4pm, ENSCBP Christian Salesse, 8 June 2017, 4pm, ENSCBP Patrice Rey, 01 June 2017, 2h30 pm, ENSCBP Christina Sizun, 18 May 2017, 4 pm, ENSCBP Marisela Velez, 5 May 2017, 11am, IECB Iqbal Choudhary, 27 April 2017, 4 pm, ENSCBP Vincent Aucagne, 21 April 2017, 11 am, IECB Sophie Zinn-Justin, 14 April 2017, 11 am, IECB Cécile Feuillie, 16 February 2017, 4 PM, ENSCBP Félix M. Goñi, 8 december 2016, 4 pm, ENSCBP Félix M. Goñi, 17 november 2016, 4 pm, ENSCBP Carl Creutz, 20 october 2016, 4 pm, ENSCBP Diego Romero, 30 september 2016, 11 am, IECB A. Ciaccafava, 8 september 2016, 14h, ENSCBP A. Ramamoorthy, 16 June 2016, 16h, ENSCBP Alexandre de Brevern, 2 june 2016, ENSCBP Isabelle Landrieu, 26 May, 16h, ENSCBP Frances Sepavoric, 12 May, 16h, ENSCBP Thomas Pradeu, 7 April 2016, 9h, ENSCBP Françoise Argoul, 3 march 2016, 16h, ENSCBP Corinne Loutelier-Bourhis, 16/12/2015, 16h Aristotelis XENAKIS, 3 december 2015, 16h Fabian Kiessling, 26 november 2015, 11h Michaël Molinari, 20 november 2015, 11h Dipankar Das Sarma, 28 October 2015, 14h. Olivier Donard, 22 october 2015, 16h, ENSCBP E. Morvan & A.Grelard, 17/12/2015, 16h, ENSCBP Christophe Cullin, 10 september 2015, 14 h Brigitte Lindet, 18 June 2015, 16h, ENSCBP(B) Marion Decossas, 4 June 2015, 16h, ENSCBP(B) Pascale Schellenberger, 21 Mai 2015, 16h F. Leal-Calderon, 7 May 2015, 16h, ENSCBP(B) Ibrahim Abdulhalim, 9 April 2015, 14h, ENSCBP Manon Carré, 26 March 2015, 14h, ENSCBP(B) C. Bure & JM Schmitter, 19 March 2015, 16h T. Ogata & H. Ihara, 17 March 2015, 11h, IECB Banafshe Larijani, 12 March 2015, 16h, ENSCBP
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Christian Griesinger, November 24th... R. Dos santos Morais, 30/11/2017, 4... Takahiro Muraoka, 16/11/2017, 11am,... Vladimir Torbeev, 9/11/2017, 2pm, E... M. D. Smith & T.Constantieux, 3/11/... James Nowick, 15 september 2017, 11... Emmanuelle Thinon, 28 June 2017, 11... Caroline Tokarski, 15 June 2017, 4p... Christian Salesse, 8 June 2017, 4pm... Patrice Rey, 01 June 2017, 2h30 pm,... Christina Sizun, 18 May 2017, 4 pm,... Marisela Velez, 5 May 2017, 11am, I... Iqbal Choudhary, 27 April 2017, 4 p... Vincent Aucagne, 21 April 2017, 11 ... Sophie Zinn-Justin, 14 April 2017, ... Cécile Feuillie, 16 February 2017,... Félix M. Goñi, 8 december 2016, 4... Félix M. Goñi, 17 november 2016, ... Carl Creutz, 20 october 2016, 4 pm,... Diego Romero, 30 september 2016, 11... A. Ciaccafava, 8 september 2016, 14... A. Ramamoorthy, 16 June 2016, 16h,... Alexandre de Brevern, 2 june 2016, ... Isabelle Landrieu, 26 May, 16h, ENS... Frances Sepavoric, 12 May, 16h, ENS... Thomas Pradeu, 7 April 2016, 9h, EN... Françoise Argoul, 3 march 2016, 1... Corinne Loutelier-Bourhis, 16/12/20... Aristotelis XENAKIS, 3 december 201... Fabian Kiessling, 26 november 2015,... Michaël Molinari, 20 november 2015... Dipankar Das Sarma, 28 October 2015... Olivier Donard, 22 october 2015, 16... E. Morvan & A.Grelard, 17/12/2015, ... Christophe Cullin, 10 september 201... Brigitte Lindet, 18 June 2015, 16h,... Marion Decossas, 4 June 2015, 16h, ... Pascale Schellenberger, 21 Mai 2015... F. Leal-Calderon, 7 May 2015, 16h, ... Ibrahim Abdulhalim, 9 April 2015, 1... Manon Carré, 26 March 2015, 14h, ... C. Bure & JM Schmitter, 19 March 20... T. Ogata & H. Ihara, 17 March 2015,... Banafshe Larijani, 12 March 2015, 1...
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Patricia DULOR Anthony BOUTER Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Yann FERRAND Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU

Patrice Rey, 01 June 2017, 2h30 pm, ENSCBP

The seminar will take place, the first of June 2017, at 2h30 pm, at the  ENSCBP amphitheater, Building B,  allée Geoffroy Saint-Hilaire, Pessac.

 

Investigating the microbiome colonizing the wood tissues of healthy and trunk-diseased grapevines

 

Pr Patrice REY

Bordeaux Sciences Agro, UMR Santé et Agroécologie du Vignoble (INRA-BSA)

 

Abstract

 

Viticulture is now confronted with vast upheavals caused by the major phenomenon of grapevine trunk diseases (GTDs), i.e. Esca and Botryosphaeria dieback in mature plants. Although GTDs, mainly Esca, have been recorded for centuries, their re-emergence within the span of a mere two decades in the late 1990s has become a subject of major concern for the viticulture sector. Vineyards throughout the world are being attacked by GTDs and, in Europe, all the table grape- or wine-producing regions (or terroirs) are affected. GTDs are caused by a broad range of taxonomically unrelated fungal pathogens such as Phaemoniella chlamydospora, Phaeoacremonium minimum, Fomitiporia mediterranea, Botryosphaeriaceae species, which attack grapevine wood tissues, inducing necroses but microbial ecology related to the disease rests still poorly understood.

 

Fungal and bacterial microflora colonizing the wood tissues of grapevines having previously expressed or not foliar symptoms of Esca were characterized and compared. Observations over a period of one year, using a fingerprinting method, Single Strand Conformation Polymorphism (SSCP), and the ITS-DNA sequencing of cultivable fungi and bacteria, showed that shifts occurred in the fungal and bacterial communities colonizing the apparently healthy wood tissues. However, whether grapevines were sampled in spring, summer, autumn or winter, the microbial communities colonizing the healthy wood tissues of asymptomatic or symptomatic plants were different. High-throughput sequencing indicated that diverse assemblages of fungi and bacteria colonise the non-necrotic tissues. Numerous potential plant-protective fungi, such as Trichoderma spp., as well as bacteria, such as Bacillus spp. and Pantoea agglomerans, were isolated from these wood tissues. Pathogenic fungi, such as Botryosphaeria spp., Phaeomoniella chlamydospora were also identified. Regarding microflora, several questions are the matter of speculation: (i) What is the role of the microbial communities that colonise the non-necrotic grapevine wood tissues? (ii) What are the interactions between fungal and bacterial communities? (iii) What are the abiotic factors that have most influence on microbial communities in the wood tissues, leading to a decrease in microbial diversity and the development of wood necroses?

 

Biography

 

Patrice Rey has been, since 2007, Professor in Plant Protection at Bordeaux Sciences Agro (France) and Deputy head of the “Vineyard Health and Agrocology” research unit INRA/BSA. Besides being a member of ANSES, devoted to “Biocontrol”, and AFPP, devoted to « Plant protection », he is a member of French Scientific Committees in various agricultural sectors: viticulture, horticulture, fruit/vegetables. At international level, he is involved in the International Organization of Vine and Wine (OIV). Since 2013, he has been a co-leader of the « Microbial Ecology » Working Group, COST FA 1303 "Sustainable control of grapevine trunk diseases". He is holder of the Industrial Chair “GTDfree” (2016-2020), funded by ANR and Maison Hennessy (LVMH group), dedicated to the control of grapevine trunk diseases.

 

Web site: https://www.maladie-du-bois-vigne.fr

 

Contact: Antoine Loquet, a.loquet@iecb.u-borddeaux.fr

 

 

 

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