Our research focuses on structural biology and biophysics of biological assemblies, ranging from functional amyloids to bacterial nanomachines and lipid membranes. We develop and mainly apply solid-state NMR spectroscopy to capture structural and dynamic details at atomic resolution.
Our group manages the NMR facility of the IECB/CBMN, including 300, 400, 500, 600, 700 and to 800MHz NMR magnets, equipped with solution and solid-state NMR probes. The 800 MHz is part of the French NMR Large Scale Facilty (TGIR RMN THC). The Bordeaux site is specialized in NMR of biological assemblies, membranes, lipids and colloid science.
Main research topics:
Amyloids are proteins that can form, under particular conditions, fibrillar aggregates (fibrils, oligomers). They are commonly associated with neurodegenerative diseases, such as Parkinson’s and Alzheimer’s diseases. Recently, such amyloid structures have been identified in programmed cell-death mechanisms. We aim at understanding the atomic architecture and study the structure-function relationship of several amyloid proteins involved in cell signaling.
SOLID-STATE NMR METHODOLOGY
Supramolecular assemblies are organized by a complex network of intermolecular interactions, controlling the assembly process and impacting the functions of the final assembled system.
We develop solid-state NMR methods to tackle complex molecular assemblies, to obtain atomic information on supramolecular interactions. Ultra-fast magic angle spinning NMR techniques are also investigated to improve sensitivity on complex biomolecular systems.
MEMBRANES AND LIPID INTERACTIONS
We use solid-state and solution NMR to study structural and dynamic aspects of membranes as well as their interactions with small organic molecules, peptides and proteins. The physical-chemistry of lipid assemblies is investigated. This includes the roles of various sterols in regulating membrane dynamics (order/disorder). Specific lipids involved in signaling (phosphoinositides, cardiolipins, DAG, …) are followed by 2H-NMR in natural membranes (viruses, tripanosomes).
We study tannin-lipid interactions in the field of oenology and health. In oenology, tannins are responsible for the astringency and the bitterness of red wines. The former implicates an interaction between tannins and saliva proteins during mouth lubrication, while the latter results from an interaction between tannins and taste receptors. We investigate tannin-lipid interactions and the anti-oxidant efficiency of tannins in membranes by liquid and solid-state NMR.
Institut Européen de Chimie et Biologie (IECB)
2 rue Robert Escarpit
Tel. : +33 (5) 40 00 30 38