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Internal CBMN Seminars: A. Baudin, ... Plateforme production de protéine... Patrick Trouillas, 21 June 2018, 2p... Lucie Khemtemourian, 21 June 2018, ... Jonathan Faherty, 7th June, 11 AM, ... Yann Fichou, 3 May 2018, 2pm, IECB Internal CBMN seminars: A. Ciaccafa... Alain Roussel, 29/03/2018, 4 pm, EN... Simon Poly, 22 march 2018, 2pm, ENS... J.Crassous, M. Sallé & A. Martinez... O.Reinaud & V. Artero, 7/12/2017, 1... R. Dos santos Morais, 30/11/2017, 4... Christian Griesinger, November 24th... Takahiro Muraoka, 16/11/2017, 11am,... Vladimir Torbeev, 9/11/2017, 2pm, E... M. D. Smith & T.Constantieux, 3/11/... James Nowick, 15 september 2017, 11... Emmanuelle Thinon, 28 June 2017, 11... Caroline Tokarski, 15 June 2017, 4p... Christian Salesse, 8 June 2017, 4pm... Patrice Rey, 01 June 2017, 2h30 pm,... Christina Sizun, 18 May 2017, 4 pm,... Marisela Velez, 5 May 2017, 11am, I... Iqbal Choudhary, 27 April 2017, 4 p... Vincent Aucagne, 21 April 2017, 11 ... Sophie Zinn-Justin, 14 April 2017, ... Cécile Feuillie, 16 February 2017,... Félix M. Goñi, 8 december 2016, 4... Félix M. Goñi, 17 november 2016, ... Carl Creutz, 20 october 2016, 4 pm,... Diego Romero, 30 september 2016, 11... A. Ciaccafava, 8 september 2016, 14... A. Ramamoorthy, 16 June 2016, 16h,... Alexandre de Brevern, 2 june 2016, ... Isabelle Landrieu, 26 May, 16h, ENS... Frances Sepavoric, 12 May, 16h, ENS... Thomas Pradeu, 7 April 2016, 9h, EN... Françoise Argoul, 3 march 2016, 1... Corinne Loutelier-Bourhis, 16/12/20... Aristotelis XENAKIS, 3 december 201... Fabian Kiessling, 26 november 2015,... Michaël Molinari, 20 november 2015... Dipankar Das Sarma, 28 October 2015... Olivier Donard, 22 october 2015, 16... E. Morvan & A.Grelard, 17/12/2015, ... Christophe Cullin, 10 september 201... Brigitte Lindet, 18 June 2015, 16h,... Marion Decossas, 4 June 2015, 16h, ... Pascale Schellenberger, 21 Mai 2015... F. Leal-Calderon, 7 May 2015, 16h, ... Ibrahim Abdulhalim, 9 April 2015, 1... Manon Carré, 26 March 2015, 14h, ... C. Bure & JM Schmitter, 19 March 20... T. Ogata & H. Ihara, 17 March 2015,... Banafshe Larijani, 12 March 2015, 1...
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Patricia DULOR Anthony BOUTER Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Yann FERRAND Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU

Alain Roussel, 29/03/2018, 4 pm, ENSCBP

The seminar will take place, the 29th of March 2018, at 4pm, at the  ENSCBP amphitheater, Building B,  allée Geoffroy Saint-Hilaire, Pessac.

 

CAMELID NANOBODIES : VERSATILE TOOLS FOR MOLECULAR AND STRUCTURAL BIOLOGY STUDIES.

 

 Dr Alain Roussel

 AFMB, CNRS and Aix-Marseille University

 163, avenue de Luminy 13288 MARSEILLE Cedex 09

 email : alain.roussel@afmb.univ-mrs.fr

 

Abstract

 

In recent years, the use of single-domain camelid immunoglobulins, termed vHHs or nanobodies, has seen increasing growth in biotechnology, pharmaceutical applications and structure/function research. The usefulness of nanobodies in molecular and structural biology is now firmly established whether to solve 3D structures or to perform functional studies. After describing our platform dedicated to the selection and production of nanobodies, I will present the recent results we have obtained in the structural studies of bacterial secretion systems (T2SS, T6SS and T9SS). Finally I will present our current work on the development of neutralizing nanobodies against Ebola virus.

 

 

Related publications

 

Desmyter, A., Spinelli, S., Roussel, A., and Cambillau, C. (2015) Camelid nanobodies: killing two birds with one stone. Current opinion in structural biology 32C, 1-8

 

Douzi, B., Trinh, N. T. T., Michel-Souzy, S., Desmyter, A., Ball, G., Barbier, P., Kosta, A., Durand, E., Forest, K. T., Cambillau, C., Roussel, A., and Voulhoux, R. (2017) Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics. MBio 8

 

Desmyter, A., Durand, E., Roussel, A., Kellenberger, C., Cascales, E., and Cambillau, C. (2017) Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol 2, 17103

 

Leone, P., Roche, J., Vincent, M. S., Tran, Q. H., Desmyter, A., Cascales, E., Kellenberger, C., Cambillau, C., and Roussel, A. (2018) Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space. Nat Commun 9, 429

 

 

Short biography

 

Alain Roussel, Director of Research CNRS. Deputy director of AFMB, Marseille, France. Head of the «Host-Pathogen Interaction » team (http://www.afmb.univ-mrs.fr/host-pathogen-interactions). Scientific coordinator of the IbiSA platform of Structural Biology at AFMB, which is supported by the French Infrastructure for Integrated Structural Biology (FRISBI). Member of the executive committee of FRISBI. Specialist of X-ray crystallography. Fields of interest: Innate immunity, Bacterial secretion systems, Monoclonal antibodies.

 

 

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