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Marc-André SELOSSE, 23 septembre 2... Christophe Bucher, Thursday July 18... Mélanie Berbon, 11 juillet 2019, 1... Hendrik W. Van Veen, July 11th, 201... Sébastien Ulrich, July 4th, 2019 ... Frédéric Brossard, 1st of July, 1... Prof. Solange Landreville, 19th of ... Prof. Hiroshi Yabu, 27th of May, 11... Amitabha Chattopadhyay, 22 May, 11 ... Prof. Hiroaki Suga, 10/05/2017, 11 ... INTERNAL CBMN SEMINAR: PHD student ... Jean Duhamel, 4 april 2019, 11am, I... Internal CBMN seminar 7 March 2019... Sandrine VIllette, 7 february 2019,... Internal CBMN Seminar : newcomers ... Internal CBMN Seminar: A. Simon, 6 ... Marisela Velez, 28 november 2018, 2... Marc Bramkamp, 16 november 2018, 2p... Kazushi Kinbara, 12 november 2018, ... Internal CBMN Seminar: J. Lang, 8 N... Internal CBMN Seminars: A. Baudin, ... Plateforme production de protéine... Patrick Trouillas, 21 June 2018, 2p... Lucie Khemtemourian, 21 June 2018, ... Jonathan Faherty, 7th June, 11 AM, ... Yann Fichou, 3 May 2018, 2pm, IECB Internal CBMN seminars: A. Ciaccafa... Alain Roussel, 29/03/2018, 4 pm, EN... Simon Poly, 22 march 2018, 2pm, ENS... J.Crassous, M. Sallé & A. Martinez... O.Reinaud & V. Artero, 7/12/2017, 1... R. Dos santos Morais, 30/11/2017, 4... Christian Griesinger, November 24th... Takahiro Muraoka, 16/11/2017, 11am,... Vladimir Torbeev, 9/11/2017, 2pm, E... M. D. Smith & T.Constantieux, 3/11/... James Nowick, 15 september 2017, 11... Emmanuelle Thinon, 28 June 2017, 11... Caroline Tokarski, 15 June 2017, 4p... Christian Salesse, 8 June 2017, 4pm... Patrice Rey, 01 June 2017, 2h30 pm,... Christina Sizun, 18 May 2017, 4 pm,... Marisela Velez, 5 May 2017, 11am, I... Iqbal Choudhary, 27 April 2017, 4 p... Vincent Aucagne, 21 April 2017, 11 ... Sophie Zinn-Justin, 14 April 2017, ... Cécile Feuillie, 16 February 2017,... Félix M. Goñi, 8 december 2016, 4... Félix M. Goñi, 17 november 2016, ... Carl Creutz, 20 october 2016, 4 pm,... Diego Romero, 30 september 2016, 11... A. Ciaccafava, 8 september 2016, 14... A. Ramamoorthy, 16 June 2016, 16h,... Alexandre de Brevern, 2 june 2016, ... Isabelle Landrieu, 26 May, 16h, ENS... Frances Sepavoric, 12 May, 16h, ENS... Thomas Pradeu, 7 April 2016, 9h, EN... Françoise Argoul, 3 march 2016, 1... Corinne Loutelier-Bourhis, 16/12/20... Aristotelis XENAKIS, 3 december 201... Fabian Kiessling, 26 november 2015,... Michaël Molinari, 20 november 2015... Dipankar Das Sarma, 28 October 2015... Olivier Donard, 22 october 2015, 16... E. Morvan & A.Grelard, 17/12/2015, ... Christophe Cullin, 10 september 201... Brigitte Lindet, 18 June 2015, 16h,... Marion Decossas, 4 June 2015, 16h, ... Pascale Schellenberger, 21 Mai 2015... F. Leal-Calderon, 7 May 2015, 16h, ... Ibrahim Abdulhalim, 9 April 2015, 1... Manon Carré, 26 March 2015, 14h, ... C. Bure & JM Schmitter, 19 March 20... T. Ogata & H. Ihara, 17 March 2015,... Banafshe Larijani, 12 March 2015, 1...
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Patricia DULOR Anthony BOUTER Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Yann FERRAND Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Caroline TOKARSKI

Amitabha Chattopadhyay, 22 May, 11 am, IECB

The seminar will take place, the 22th of May, at 11 am in the  IECB amphitheatre, 2 rue Robert Escarpit Pessac.

 

Cholesterol-induced Conformational Plasticity and Oligomerization of GPCRs: Novel Insights in Health and Disease

 

Prof. Amitabha Chattopadhyay

Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, India

amit@ccmb.res.in

URL: http://e-portal.ccmb.res.in/e-space/amit/Pages/Index.htm

 

Contact: Isabel Alves, i.alves@cbmn.u-bordeaux.fr

 

Abstract

 

G protein-coupled receptors (GPCRs) are the largest class of molecules involved in signal transduction across membranes, and represent major drug targets in all clinical areas. The overall focus of our work is on the role of membrane cholesterol in GPCR organization (oligomerization), dynamics and function with implications in health and disease. The GPCR of choice is the serotonin1A receptor, an important neurotransmitter receptor implicated in the generation and modulation of cognitive, behavioral and developmental functions, and an important drug target.

 

We demonstrated that membrane cholesterol is necessary for ligand binding, and G-protein coupling and signaling of serotonin1A receptors. Interestingly, high-resolution crystal structures of GPCRs exhibit bound cholesterol. In this context, we reported the presence of cholesterol recognition/interaction amino acid consensus (CRAC) motifs in the serotonin1A receptor. Recent results employing mutations in the CRAC motifs show the importance of Lys101 in transmembrane helix II in conferring cholesterol-sensitivity to signaling by the receptor. In addition, using a combination of experimental and MD simulation approaches, we demonstrated that the receptor is more stable and compact in the presence of membrane cholesterol. Further, our results provide important insights in cholesterol-dependent oligomerization of the receptor using a variety of approaches such as photobleaching homo-FRET, photobleaching image correlation spectroscopy, and coarse grain MD simulations. We envision that progress in deciphering molecular details of the nature of GPCR-cholesterol interaction in the membrane would lead to better insight into our overall understanding of GPCR function in health and disease.

 

Biography

 

Prof. Amitabha Chattopadhyay received B.Sc. with Honors in Chemistry from St. Xavier’s College (Calcutta) and M.Sc. from IIT Kanpur. He obtained his Ph.D. from the State University of New York (SUNY) at Stony Brook, and was a Postdoctoral Fellow at the University of California, Davis. He subsequently joined the Centre for Cellular and Molecular Biology (CCMB) in Hyderabad and now is a SERB Distinguished Fellow there.

 

Prof. Chattopadhyay’s work is focused on monitoring organization, dynamics and function of biological membranes in healthy and diseased conditions. His group has developed and applied novel, innovative and sensitive techniques (such as the wavelength-selective fluorescence approach) using fluorescence spectroscopy for monitoring solvent relaxation in membranes, membrane-mimetic media, and proteins. These pioneering studies have led to a better understanding ofthe dynamics of hydration of membranes and proteins. Another seminal contribution of Prof. Chattopadhyay’s group focuses on the role of membrane cholesterol in regulating the organization, dynamics and function of G protein-coupled receptors such as the serotonin1A receptor. His work showed, for the first time, that membrane cholesterol is necessary for the function of Gprotein-coupled receptors (GPCRs) such as the serotonin1A receptor. His work has also provided novel insight in the role of membrane cholesterol in the entry of pathogens into host cells. Prof. Chattopadhyay has used fluorescence-based microscopic approaches such as Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Correlation Spectroscopy (FCS), and Fluorescence Resonance Energy Transfer (FRET) to provide novel insight into organization, dynamics and function of membrane-bound receptors. Overall, his work has contributed significantly to the understanding of membrane organization and dynamics, and the interplay between membrane lipids and proteins, especially in neuronal membranes.

 

Prof. Chattopadhyay was awarded the prestigious The World Academy of Sciences (TWAS) Prize, Shanti Swarup Bhatnagar Award,Ranbaxy Research Award, Prof. G.N. Ramachandran 60th Birthday Medal from the Indian National Science Academy, SERB Distinguished Fellowship, and J.C. Bose Fellowshipfrom the Dept. of Science and Technology, Govt. of India. He is an elected Fellow of Fellow of The World Academy of Sciences, Royal Society of Biology, RoyalSociety of Chemistry, and all the Indian Academies of Science. Prof. Chattopadhyay has served on the editorial boards of a large number of journals that include Biophysical Journal,The Journal of Physical Chemistry, Journal of Neurochemistry, BBA-Biomembranes, Journal of Membrane Biology, FEBS Letters, IUBMB Lifeand ACS Chemical Neuroscience. He has mentored a number of students for Ph.D. Prof. Chattopadhyay has authored close to 300 research publications (mostly as first or senior/corresponding author; total citations > 11,300, h-index57, i-10index 188), two monographs, and national and international patents. He has delivered close to 600 invited lectures all over the world including keynote, plenary, and colloquium lectures. Prof. Chattopadhyay has organized a number of international conferences on the broad theme of biological membranes including a thematic meeting of the Biophysical Society. Prof. Chattopadhyay has been instrumental in designing and teaching courses related to biomembranes and fluorescence spectroscopy for Ph.D. students in India and abroad. Prof. Chattopadhyay is involved with science awareness and popularization programs among high school and college studentsin India, and in giving lectures to students and junior faculty on manuscript and grant writing. Prof. Chattopadhyay is a Distinguished Visiting Professor at the Indian Institute of Technology Bombay (Mumbai), Adjunct Professor at theJawaharlalNehru University (New Delhi), Tata Institute of Fundamental Research (Mumbai), Indian Institute of Science Education and Research (Kolkata), Royal Melbourne Institute of Technology (Australia), Swinburne University of Technology (Australia), and Honorary Professor at the Jawaharlal Nehru Centre for Advanced Scientific Research (Bangalore).He servedas the first Dean of Biological Sciences of the Academy ofScientific and Innovative Research.

 

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