Institut de Chimie & Biologie des Membranes & des Nano-objets • Bordeaux

LIA France-Japan GEM Réseau RMN Aquitain Master AC2QMAPS IPEP master UREKA UMT FOLIES INDIA
Offres d'Emploi Plateau Technique Conseil d'Unité Animation Scientifique WEB Editorial Committee Hygiène & Sécurité Communication Administration Directoire Scientifique Direction
O.Reinaud & V. Artero, 7/12/2017, 10 am, IECB R. Dos santos Morais, 30/11/2017, 4 PM, ENSCBP Christian Griesinger, November 24th, 11am, IECB Takahiro Muraoka, 16/11/2017, 11am, ENSCBP Vladimir Torbeev, 9/11/2017, 2pm, ENSCBP M. D. Smith & T.Constantieux, 3/11/2017, 2h30 pm, IECB James Nowick, 15 september 2017, 11am, IECB Emmanuelle Thinon, 28 June 2017, 11 am, IECB Caroline Tokarski, 15 June 2017, 4pm, ENSCBP Christian Salesse, 8 June 2017, 4pm, ENSCBP Patrice Rey, 01 June 2017, 2h30 pm, ENSCBP Christina Sizun, 18 May 2017, 4 pm, ENSCBP Marisela Velez, 5 May 2017, 11am, IECB Iqbal Choudhary, 27 April 2017, 4 pm, ENSCBP Vincent Aucagne, 21 April 2017, 11 am, IECB Sophie Zinn-Justin, 14 April 2017, 11 am, IECB Cécile Feuillie, 16 February 2017, 4 PM, ENSCBP Félix M. Goñi, 8 december 2016, 4 pm, ENSCBP Félix M. Goñi, 17 november 2016, 4 pm, ENSCBP Carl Creutz, 20 october 2016, 4 pm, ENSCBP Diego Romero, 30 september 2016, 11 am, IECB A. Ciaccafava, 8 september 2016, 14h, ENSCBP A. Ramamoorthy, 16 June 2016, 16h, ENSCBP Alexandre de Brevern, 2 june 2016, ENSCBP Isabelle Landrieu, 26 May, 16h, ENSCBP Frances Sepavoric, 12 May, 16h, ENSCBP Thomas Pradeu, 7 April 2016, 9h, ENSCBP Françoise Argoul, 3 march 2016, 16h, ENSCBP Corinne Loutelier-Bourhis, 16/12/2015, 16h Aristotelis XENAKIS, 3 december 2015, 16h Fabian Kiessling, 26 november 2015, 11h Michaël Molinari, 20 november 2015, 11h Dipankar Das Sarma, 28 October 2015, 14h. Olivier Donard, 22 october 2015, 16h, ENSCBP E. Morvan & A.Grelard, 17/12/2015, 16h, ENSCBP Christophe Cullin, 10 september 2015, 14 h Brigitte Lindet, 18 June 2015, 16h, ENSCBP(B) Marion Decossas, 4 June 2015, 16h, ENSCBP(B) Pascale Schellenberger, 21 Mai 2015, 16h F. Leal-Calderon, 7 May 2015, 16h, ENSCBP(B) Ibrahim Abdulhalim, 9 April 2015, 14h, ENSCBP Manon Carré, 26 March 2015, 14h, ENSCBP(B) C. Bure & JM Schmitter, 19 March 2015, 16h T. Ogata & H. Ihara, 17 March 2015, 11h, IECB Banafshe Larijani, 12 March 2015, 16h, ENSCBP
Événements Nouveau
Partenariat Congés Formation Admin CBMN Conseil Institut/Scientifique
Affiliations Les sites CBMN
LIA France-Japan GEM Réseau RMN Aquitain Master AC2QMAPS IPEP master UREKA UMT FOLIES INDIA
Offres d'Emploi Plateau Technique Conseil d'Unité Animation Scientifique WEB Editorial Committee Hygiène & Sécurité Communication Administration Directoire Scientifique Direction
Chimie Biophysique Chimie Biomimétique et Thérapeuti... Biologie et Biotechnologie Administration
O.Reinaud & V. Artero, 7/12/2017, 1... R. Dos santos Morais, 30/11/2017, 4... Christian Griesinger, November 24th... Takahiro Muraoka, 16/11/2017, 11am,... Vladimir Torbeev, 9/11/2017, 2pm, E... M. D. Smith & T.Constantieux, 3/11/... James Nowick, 15 september 2017, 11... Emmanuelle Thinon, 28 June 2017, 11... Caroline Tokarski, 15 June 2017, 4p... Christian Salesse, 8 June 2017, 4pm... Patrice Rey, 01 June 2017, 2h30 pm,... Christina Sizun, 18 May 2017, 4 pm,... Marisela Velez, 5 May 2017, 11am, I... Iqbal Choudhary, 27 April 2017, 4 p... Vincent Aucagne, 21 April 2017, 11 ... Sophie Zinn-Justin, 14 April 2017, ... Cécile Feuillie, 16 February 2017,... Félix M. Goñi, 8 december 2016, 4... Félix M. Goñi, 17 november 2016, ... Carl Creutz, 20 october 2016, 4 pm,... Diego Romero, 30 september 2016, 11... A. Ciaccafava, 8 september 2016, 14... A. Ramamoorthy, 16 June 2016, 16h,... Alexandre de Brevern, 2 june 2016, ... Isabelle Landrieu, 26 May, 16h, ENS... Frances Sepavoric, 12 May, 16h, ENS... Thomas Pradeu, 7 April 2016, 9h, EN... Françoise Argoul, 3 march 2016, 1... Corinne Loutelier-Bourhis, 16/12/20... Aristotelis XENAKIS, 3 december 201... Fabian Kiessling, 26 november 2015,... Michaël Molinari, 20 november 2015... Dipankar Das Sarma, 28 October 2015... Olivier Donard, 22 october 2015, 16... E. Morvan & A.Grelard, 17/12/2015, ... Christophe Cullin, 10 september 201... Brigitte Lindet, 18 June 2015, 16h,... Marion Decossas, 4 June 2015, 16h, ... Pascale Schellenberger, 21 Mai 2015... F. Leal-Calderon, 7 May 2015, 16h, ... Ibrahim Abdulhalim, 9 April 2015, 1... Manon Carré, 26 March 2015, 14h, ... C. Bure & JM Schmitter, 19 March 20... T. Ogata & H. Ihara, 17 March 2015,... Banafshe Larijani, 12 March 2015, 1...
Événements Nouveau
Patricia DULOR Anthony BOUTER Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Yann FERRAND Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU

Plateformes

Platforms are opened to Academic & Industrial partners

(Information on access and quotation may be obtained through the local contact (Corr.))

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Nuclear Magnetic Resonance (with UMS 3033, IECB, and UPR 9048, ICMCB)

BORDEAUX SITE, "MEMBRANE & COLLOID SCIENCE", FOR THE FRENCH LARGE SCALE HIGH FIELD NMR FACILITY (TGIR RMN THC)

Resp. Erick Dufourc (e.dufourc@cbmn.u-bordeaux.fr, Tel: +33 5 4000 6818)

Corr. Axelle Grélard (a.grelard@iecb.u-bordeaux.fr, Tel: +33 5 4000 2230) & Estelle Morvan (IECB, e.morvan@iecb.u-bordeaux.frTel: +33 5 4000 2230)

EQUIPMENT

Spectrometer 800 MHz for NMR in Liquid, Solid & Soft matter

(Probes: Cryoprobe 5 mm TCI (1H/13C/15N/2H) with Z-gradient; CPMAS 4 mm (1H/BB); CPMAS 3.2 mm (1H/13C/15N))

Spectrometer 700 MHz for NMR in Liquid, Solid & Soft matter

(Probes: TXI 5 mm (1H/13C/15N/2H), Z-gradient; BBO 5 mm (109Ag – 31P/1H/2H), Z-gradient; HRMAS 4 mm (15N – 31P/1H); CPMAS 4 mm (1H/2H/31P), E-free; CPMAS 4 mm (15N – 31P/1H))

Spectrometer 600 MHz for NMR in Solid & Soft matter

(Probes: Quadruple resonance 1.3 mm CPMAS (1H/13C/15N/2H); BioSolids Triple Resonance 4mm and 3.2 mm CPMAS (1H/13C/15N); Dual Resonance 4 mm CPMAS (1H-19F/15N-31P))

Spectrometer 500 MHz for NMR in Liquid, Solid & Soft matter

(Probes: CPMAS 2,5 mm, (νr ≤ 33 kHz) (1H/X/Y); 2x CPMAS 4 mm (1H/X)

Spectrometer 400 MHz for NMR in Liquid state

(Probes: 5 mm « SmartProbe » BBFO (109Ag-31P-19F/1H/2H) Z-gradient; QNP 5 mm (13C/31P/19F/1H/2H), Z-gradient; BBI 5 mm (15N-31P/1H/2H), Z-gradient)

Spectrometer 300 MHz for NMR in Solid state

(Probes: CPMAS 4 mm (1H/X/Y); CPMAS 4 mm (1H/X/Y), high temperatures (< 300°C)); WL 4 & 7 mm Gonio automatic (1H/X/Y))

Spectrometer 300 MHz for NMR in Liquid state

(Probe: BBFO 5 mm (15N-31P/1H/2H), Z-gradient)

Spectrometer 100 MHz for NMR in Solid state

(Probe: MAS 2.5 mm (X))

  • Measurements: All NMR experiments you can think of (and that you never dared to ask)

 

Atomic Force Microscopy

Resp. Jean-Pierre Aimé (jp.aime@cnanogso.org, Tel:+33 5 4000 6846)
Corr. Touati Douar (t.douar@cbmn.u-bordeaux.fr, Tel: +33 5 4000 6834)

EQUIPMENT

Atomic Force Microscope  FAstScan

Atomic Force Microscope  Asylum 3D

Laboratories for Single Wall & Multiple Wall Carbon Nanotubes synthesis and facility for Carbon Nanotubes soldering on cantilever tip

 

X-Rays Diffraction/Diffusion (with UMS 3033, IECB)

Resp. Ivan Huc (i.huc@iecb.u-bordeaux.fr, Tel:+33 5 4000 2219)
Corr. Brice Kauffmann (b.kauffmann@iecb.u-bordeaux.fr, Tel: +33 5 4000 3054)

EQUIPMENT

Rotating Anode Rigaku MM07 HF 1,2 kW

Rotating Anode Rigaku FRX 3kW with microfocus (Hybrid Dectris Pilatus 200 K pixel detector)

  • Mesurement of diffracted intensities on monocristals of organic/biologic molecules and macromolecules (proteins, nucleix acids, macromolecular complexes...) and determination of their 3D structure.
  • Mesurement of X-rays scattering and diffusion at small and wide angles with diffusion factor ranges, q = 0.08 à 3 Å-1. Low resolution structure determination, molecular enveloppes (SAXS, WAXS)
  • Mesurement of diffuse scattering on monocristals.

Vibrational Spectroscopy

Resp. Sophie Lecomte (s.lecomte@cbmn.u-bordeaux.fr, Tel:+33 5 4000 6849)
Corr. Sabine Castano (s.castano@cbmn.u-bordeaux.fr, Tel: +33 5 4000 68 48)

EQUIPMENT


WITec Alpha 300 Raman Spectrometer

Available wavelengths: 532 and 633 nm
Measurement modes:
   Confocal Raman Imaging; Confocal Rayleigh Imaging in transmission and reflection; Atomic Force Microscopy (contact and tapping) coupled with optical techniques; Near-field Scanning Optical Microscopy (NSOM) in transmission

Bruker Hyperion 3000 FTIR Microscope

Detectors: Single element MCT; Focal Plane Array 64x64
Spectrometer: Tensor 27
Measurement modes:
    Reflection (15x and 36x objectives); Transmission (15x); 20x Attenuated Total Reflection (ATR)objective, sensing surface of 200 um,  Germanium cristal

FTIR spectrometers (1 Nicolet 6700, Nicolet  nexus 870)

Measurement modes:
    ATR Germanium; ATR Germanium with controlled temperature; ATR Diamond "Golden Gate"

PMIRRAS FTIR Setup (homebuilt)

    Coupled  with Nima Langmuir-Blodgett Troughs
    MCT Detector

Ellipsometer NFT Nanofilm Surface analysis

    Wavelength: 532nm
    Coupled  with Nima Langmuir-Blodgett Troughs

Plasmon Waveguide Resonance Spectrometer (1 Proterion prototype, 1 homebuilt)

Available wavelengths: 541 and 633 nm
Key points:
    Sensitive to anisotropy (s- and p-polarization)
    Automated Acquisition
    Fast acquisition for kinectics characterization
    Binding affinities from pM to mM
    Label free technique
Function:
    Study the interaction of a protein with a lipid membrane formed on the biosensor surface.

 

Mass Spectrometry (with CGFB)

 

AS PART OF THE PROTEOME/LIPIDOME PLATFORM (CGFB/PROTEOME)

 

Resp. Jean-Marie Schmitter (jm.schmitter@cbmn.u-bordeaux.fr, Tel:+33 5 5757 5624)
Corr. Katel Bathany (k.bathany@cbmn.u-bordeaux.fr, Tel: +33 5 5757 5622) & Corinne Buré (c.bure@cbmn.u-bordeaux.fr, Tel: +33 5757 5623)

 

EQUIPMENT

  • Q-Exactive

    U3000-RSLC + Q-Exactive (Thermo Scientific).
  • LTQ Orbitrap XL

    U3000 (Dionex)/Automate HDX (LEAP) + LTQ Orbitrap XL ETD (Thermo Scientific).
  • MALDI-TOF Mass Spectrometer

    Ultraflex III TOF-TOF (Bruker Daltonics) + High Mass Detector HM2 (CovalX).
  • Mass spectrometry imaging

    Preparation Robot ImagePrep (Bruker Daltonics).
  • Q-TOF Premier

    U3000-RSLC (Thermo Scientific) + Q-TOF premier (Waters Micromass).

 

 

 

Cryo-Electron Microscopy (with UMS 3033, IECB)

Resp. Alain Brisson (a.brisson@cbmn.u-bordeaux.fr, Tel:+33 5 4000 6861)

Olivier Lambert (o.lambert@cbmn.u-bordeaux.fr, Tel:+33 5 4000 6829)

Rémi Fronzes (r.fronzes@iecb.u-bordeaux.fr)

Facility Engineers Marion Decossas (m.decossas@cbmn.u-bordeaux.fr, Tel: +33 5 4000 6811)

Sisareuth Tan (s.tan@cbmn.u-bordeaux.fr, Tel: +33 5 4000 6859)

Armel Bezault (a.bezault@iecb.u-bordeaux.fr)

 

SERVICES AND EXPERTISE

 

Samples preparation for MET and Cryo-MET experiments

Preparation of biological samples and synthetic, organic and metallic assemblies

Sub-cellular preparation of proteins, protein-membrane complexes : negative staining, plunge freezing

MET, cryo-EM and Tomography of biological samples, inorganic nanoparticles, polymers, natives or functionalized

EQUIPMENT

Transmission electron microscope: Tecnai-F20 200kV-FEG (FEI)

(Ambient or cryo-TEM – 2k_2k USC1000 slow-scan CCD camera GATAN)

Transmission electron microscope: CM-120 120 kV (FEI)

(Ambient or cryo-TEM – 2k_2k USC1000 slow-scan CCD camera GATAN)

Talos Arctica 200kV-FEG (FEI) being installed

  • SAMPLE PREPARATION for TEM observation

    • At ambient and low temperature (cryo-TEM)

    • Biological samples and organic and metallic synthetic assemblies.

    • Tissus & cells: embedding in resins and ultramicrotomy.

    • Sub-cellular preparations, proteins, membrane-protein complexes: negative staining and cryo-TEM of thin films.

  • TOMOGRAPHY of biological samples, inorganic nanoparticles or polymers (ambient temperature and cryo-TEM)

  • Atomic force microscopy (AFM) of functionalized supports for nanobiotechnology applications.

ACCESS

For any analytical experiment with assistance or free access, or for a R&D project, please ask the facility engineers for a detailed quotation

 

Peptide Synthesis

Resp. & Corr. Claude Manigand (c.manigand@cbmn.u-bordeaux.fr, Tel:+33 5 4000 6827)

 

EQUIPMENT

CEM automatic MicroWave Peptide Synthesizer

Applied Biosystems Peptide Synthesizer 431A

Analytical 600E Waters HPLC

Preparative 510 Waters HPLC

2 Analytical & Preparative Alliance Waters HPLC

Dura dry freeze-drying apparatus

  • Synthesis on the hundred mg scale
  • Length up to 60 aminoacids
  • Scale ranging between 0.1 mmole to 5 mmole
  • Amphiphiiic and hydrophobic peptides
  • Purity greather than 98%

 

Molecular Modelling

Resp. Isabelle Bestel (isabelle.berque-bestel@u-bordeaux.fr, +33 5 4000 6853)
Corr. Jean Dessolin (j.dessolin@cbmn.u-bordeaux.fr, +33 5 4000 6810)

EQUIPMENT

8 calculation blades amounting for >200 CPUs

1 Workstation with 24 CPUs and 4 NVidia GPU ~10,000 CUDA cores

Individual Graphic Stations (terminals)

SOFTWARES

Accelrys Discovery Studio 3.1 (general use)

Maestro (general use and conformational search)

Gold (docking)

KNOW-HOW

Molecular Dynamics, all atom and coarse grain (Gromacs)

Virtual Screening and Drug Design (vina, autodock, dock…)

  • Membrane simulations
  • Protein-Protein and Protein-DNA interactions
  • Small molecules self assembly (vesicles, micelles, bilayer…)
  • Virtual chemical database generation for high throughput screening
  • Protein reconstruction and mutations

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EXT INDIA UMT FOLIES UREKA IPEP master Master AC2QMAPS Réseau RMN Aquitain GEM LIA France-Japan
Équipes Patricia DULOR Anthony BOUTER Olivier LAMBERT Jochen LANG Pascale SUBRA-PATERNAULT Maria URDACI Marie-Christine DURRIEU Xavier SANTARELLI Gilles GUICHARD Yann FERRAND Isabelle BESTEL Juan ELEZGARAY Antoine LOQUET Sophie LECOMTE Reiko ODA Marc BONNEU
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Dernière mise à jour : 08/12/2017

CNRS Université de Bordeaux Institut Polytechnique de Bordeaux

CBMN Allée de Geoffroy St Hilaire Bât B14
33600 Pessac
Tél. : +33 (0)5 40 00 68 00
Fax : +33 (0)5 40 00 22 00
Mail : contact@cbmn.u-bordeaux.fr