Architecture de complexes membranaires et processus cellulaires

Dir. : Olivier LAMBERT

 

Le groupe s’intéresse à l’architecture de complexes membranaires dans des systèmes simplifiés de type protéines –liposome et également dans la cellule.

Nos centres d’intérêt actuellement sont (i) l’assemblage des pompes à efflux multidrogues chez Pseudomonas aeruginosa , (ii) la formation des jonctions adhérentes interendothéliales à base de cadhérines, (iii) les interactions virus cellule-hôte (iv) la vectorisation de molécules actives.

Notre recherche s’appuie sur deux méthodes d’imagerie: la cryotomographie électronique (film mince pour les complexes protéiques reconstitués en membrane et CEMOVIS pour la cellule) et la microscopie de fluorescence. Nos cherchons également à développer des nano-marqueurs fonctionnalisés détectables par microscopie électronique.

 

Equipe :

Pierre BONNAFOUS Elise DARGELOS Laetitia DAURY JOUCLA
Marion DECOSSAS Audrey ETCHEGOIN Marie-Christine GERBOD-GIANNONE
Olivier LE BIHAN Marion LEFEVRE Alexis MOUGEOLLE
Grégoire NAUDIN Sylvie POUSSARD Jean-Christophe TAVEAU
Xuejiao YIN

Publications :

1 - Desert, A.; Chaduc, I.; Fouilloux, S.; Taveau, J. C.; Lambert, O.; Lansalot, M.; Bourgeat-Lami, E.; Thill, A.; Spalla, O.; Ravaine, S.; Duguet, E.
High-yield preparation of polystyrene/silica clusters of controlled morphology.
Polymer Chemistry, 2012, 3, 1130-1132.
-Abstract-
2 - Hess, E.; Duheron, V.; Decossas, M.; Lezot, F.; Berdal, A.; Chea, S.; Golub, R.; Bosisio, M. R.; Bridal, S. L.; Choi, Y.; Yagita, H.; Mueller, C. G.
RANKL induces organized lymph node growth by stromal cell proliferation.
Journal of Immunology, 2012, 188, 1245-54.
3 - Mevel, M.; Sainlos, M.; Chatin, B.; Oudrhiri, N.; Hauchecorne, M.; Lambert, O.; Vigneron, J. P.; Lehn, P.; Pitard, B.; Lehn, J. M.
Paromomycin and neomycin B derived cationic lipids: Synthesis and transfection studies.
Journal of Controlled Release, 2012, 158, 461-469.
-Abstract-
4 - Perche, F.; Lambert, O.; Berchel, M.; Jaffres, P. A.; Pichon, C.; Midoux, P.
Gene transfer by histidylated lipopolyplexes: A dehydration method allowing preservation of their physicochemical parameters and transfection efficiency.
International Journal of Pharmaceutics, 2012, 423, 144-150.
-Abstract-
5 - Ta, H. P.; Berthelot, K.; Coulary-Salin, B.; Castano, S.; Desbat, B.; Bonnafous, P.; Lambert, O.; Alves, I.; Cullin, C.; Lecomte, S.
A yeast toxic mutant of HET-s amyloids disrupts membrane integrity.
Biochem. Biophys. Acta, 2012, 1818, 2325-2334.
6 - Bechetoille, N.; Vachon, H.; Gaydon, A.; Boher, A.; Fontaine, T.; Schaeffer, E.; Decossas, M.; Andre-Frei, V.; Mueller, C. G.
A new organotypic model containing dermal-type macrophages.
Experimental Dermatology, 2011, 20, 1035-1037.
-Abstract-
7 - Chevre, R.; Le Bihan, O.; Beilvert, F.; Chatin, B.; Barteau, B.; Mevel, M.; Lambert, O.; Pitard, B.
Amphiphilic block copolymers enhance the cellular uptake of DNA molecules through a facilitated plasma membrane transport.
Nucleic Acids Research, 2011, 39, 1610-1622.
-Abstract-
8 - Dieker, J. W.; Iglesias-Guimarais, V.; Decossas, M.; Yuste, V. J.; Muller, S.
Early apoptodic reorganization of spliceosomal proteins involves caspases, CAD and rearrangement of NuMA.
Traffic, 2011, , .
9 - Duguet, E.; Desert, A.; Perro, A.; Ravaine, S.
Design and elaboration of colloidal molecules: an overview.
Chemical Society Reviews, 2011, 40, 941-960.
-Abstract-
10 - Duheron, V.; Hess, E.; Duval, M.; Decossas, M.; Castaneda, B.; Klopper, J. E.; Amoasii, L.; Barbaroux, J. B.; Williams, I. R.; Yagita, H.; Penninger, J.; Choi, Y. W.; Lezot, F.; Groves, R.; Paus, R.; Mueller, C. G.
Receptor activator of NF-kappa B (RANK) stimulates the proliferation of epithelial cells of the epidermo-pilosebaceous unit.
Proceedings of the National Academy of Sciences of the United States of America, 2011, 108, 5342-5347.
-Abstract-
11 - Jacquemet, A.; Lemiegre, L.; Lambert, O.; Benvegnu, T.
How the Stereochemistry of a Central Cyclopentyl Ring Influences the Self-Assembling Properties of Archaeal Lipid Analogues: Synthesis andl CryoTEM Observations.
Journal of Organic Chemistry, 2011, 76, 9738-9747.
-Abstract-
12 - Le Bihan, O.; Chevre, R.; Mornet, S.; Garnier, B.; Pitard, B.; Lambert, O.
Probing the in vitro mechanism of action of cationic lipid/DNA lipoplexes at a nanometric scale.
Nucleic Acids Research, 2011, 39, 1595-1609.
-Abstract-
13 - Meyre, M. E.; Raffard, G.; Franconi, J. M.; Duguet, E.; Lambert, O.; Faure, C.
Production of magnetic multilamellar liposomes as highly T(2)-efficient MRI contrast agents.
Nanomedicine-Nanotechnology Biology and Medicine, 2011, 7, 18-21.
-Abstract-
14 - Meyre, M. E.; Raffard, G.; Franconi, J. M.; Duguet, E.; Lambert, O.; Faure, C.
Production of magnetic multilamellar liposomes as highly T-2-efficient MRI contrast agents.
Nanomedicine-Nanotechnology Biology and Medicine, 2011, 7, 18-21.
-Abstract-
15 - Page, N.; Gros, F.; Schall, N.; Decossas, M.; Bagnard, D.; Briand, J. P.; Muller, S.
HSC70 blockade by the therapeutic peptide P140 affects autophagic processes and endogenous MHCII presentation in murine lupus.
Annals of the Rheumatic Diseases, 2011, 70, 837-843.
-Abstract-
16 - Roger, B.; Papin, J.; Vacher, P.; Raoux, M.; Mulot, A.; Dubois, M.; Kerr-Conte, J.; Voy, B. H.; Pattou, F.; Charpentier, G.; Jonas, J. C.; Moustaid-Moussa, N.; Lang, J.
Adenylyl cyclase 8 is central to glucagon-like peptide 1 signalling and effects of chronically elevated glucose in rat and human pancreatic beta cells.
Diabetologia, 2011, 54, 390-402.
-Abstract-
17 - Almagro, S.; Durmort, C.; Chervin-Petinot, A.; Heyraud, S.; Dubois, M.; Lambert, O.; Maillefaud, C.; Hewat, E.; Schaal, J. P.; Huber, P.; Gulino-Debrac, D.
The Motor Protein Myosin-X Transports VE-Cadherin along Filopodia To Allow the Formation of Early Endothelial Cell-Cell Contacts.
Molecular and Cellular Biology, 2010, 30, 1703-1717.
-Abstract-
18 - Ben-Valid, S.; Dumortier, H.; Decossas, M.; Sfez, R.; Meneghetti, M.; Bianco, A.; Yitzchaik, S.
Polyaniline-coated single-walled carbon nanotubes: synthesis, characterization and impact on primary immune cells.
Journal of Materials Chemistry, 2010, 20, 2408-2417.
-Abstract-
19 - Bonnafous, P.; Perrault, M.; Le Bihan, O.; Bartosch, B.; Lavillette, D.; Penin, F.; Lambert, O.; Pecheur, E. I.
Characterization of hepatitis C virus pseudoparticles by cryo-transmission electron microscopy using functionalized magnetic nanobeads.
Journal of General Virology, 2010, 91, 1919-1930.
-Abstract-
20 - Brule, C.; Dargelos, E.; Diallo, R.; Listrat, A.; Bechet, D.; Cottin, P.; Poussard, S.
Proteomic study of calpain interacting proteins during skeletal muscle aging.
Biochimie, 2010, 92, 1923-1933.
-Abstract-
21 - Claudon, P.; Violette, A.; Lamour, K.; Decossas, M.; Fournel, S.; Heurtault, B.; Godet, J.; Mely, Y.; Jamart-Gregoire, B.; Averlant-Petit, M. C.; Briand, J. P.; Duportail, G.; Monteil, H.; Guichard, G.
Consequences of Isostructural Main-Chain Modifications for the Design of Antimicrobial Foldamers: Helical Mimics of Host-Defense Peptides Based on a Heterogeneous Amide/Urea Backbone.
Angewandte Chemie-International Edition, 2010, 49, 333-336.
22 - Dargelos, E.; Brule, C.; Stuelsatz, P.; Mouly, V.; Veschambre, P.; Cottin, P.; Poussard, S.
Up-regulation of calcium-dependent proteolysis in human myoblasts under acute oxidative stress.
Experimental Cell Research, 2010, 316, 115-125.
-Abstract-
23 - Fouilloux, S.; Desert, A.; Tache, O.; Spalla, O.; Daillant, J.; Thill, A.
SAXS exploration of the synthesis of ultra monodisperse silica nanoparticles and quantitative nucleation growth modeling.
Journal of Colloid and Interface Science, 2010, 346, 79-86.
-Abstract-
24 - Illien, F.; Finet, S.; Lambert, O.; Ayala-Sanmartin, J.
Different molecular arrangements of the tetrameric annexin 2 modulate the size and dynamics of membrane aggregation.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 1790-1796.
-Abstract-
25 - Lacotte, S.; Dumortier, H.; Decossas, M.; Briand, J. P.; Muller, S.
Identification of New Pathogenic Players in Lupus: Autoantibody-Secreting Cells Are Present in Nephritic Kidneys of (NZBxNZW)F1 Mice.
Journal of Immunology, 2010, 184, 3937-3945.
-Abstract-
26 - Lamaziere, A.; Maniti, O.; Wolf, C.; Lambert, O.; Chassaing, G.; Trugnan, G.; Ayala-Sanmartin, J.
Lipid domain separation, bilayer thickening and pearling induced by the cell penetrating peptide penetratin.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 2223-2230.
-Abstract-
27 - Meyre, M. E.; Clerac, R.; Mornet, S.; Duguet, E.; Dole, F.; Nallet, F.; Lambert, O.; Trepout, S.; Faure, C.
Multilamellar liposomes entrapping aminosilane-modified maghemite nanoparticles: "magnetonions''.
Physical Chemistry Chemical Physics, 2010, 12, 12794-12801.
-Abstract-
28 - Pecheur, E. I.; Diaz, O.; Molle, J.; Icard, V.; Bonnafous, P.; Lambert, O.; Andre, P.
Morphological Characterization and Fusion Properties of Triglyceride-rich Lipoproteins Obtained from Cells Transduced with Hepatitis C Virus Glycoproteins.
Journal of Biological Chemistry, 2010, 285, 25802-25811.
-Abstract-
29 - Roumes, H.; Leloup, L.; Dargelos, E.; Brustis, J. J.; Daury, L.; Cottin, P.
Calpains: Markers of tumor aggressiveness?.
Experimental Cell Research, 2010, 316, 1587-1599.
-Abstract-
30 - Stuelsatz, P.; Pouzoulet, F.; Lamarre, Y.; Dargelos, E.; Poussard, S.; Leibovitch, S.; Cottin, P.; Veschambre, P.
Down-regulation of MyoD by Calpain 3 Promotes Generation of Reserve Cells in C2C12 Myoblasts.
Journal of Biological Chemistry, 2010, 285, 12670-12683.
-Abstract-
31 - Trepout, S.; Taveau, J. C.; Benabdelhak, H.; Granier, T.; Ducruix, A.; Frangakis, A. S.; Lambert, O.
Structure of reconstituted bacterial membrane efflux pump by cryo-electron tomography.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 1953-1960.
-Abstract-
32 - Trepout, S.; Taveau, J.-C.; Lambert, O.
Structure determination of membrane protein by both cryo-electron tomography and single particle analysis.
Methods Mol Biol, 2010, 654, 207-20.
-Abstract-
33 - Vie, V.; Legardinier, S.; Chieze, L.; Le Bihan, O.; Qin, Y.; Sarkis, J.; Hubert, J. F.; Renault, A.; Desbat, B.; Le Rumeur, E.
Specific anchoring modes of two distinct dystrophin rod sub-domains interacting in phospholipid Langmuir films studied by atomic force microscopy and PM-IRRAS.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 1503-1511.
-Abstract-
CNRS-BordeauxI-IPB

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