Spectroscopies Vibrationnelles et Propriétés Optiques de Systèmes Biochimiques

Dir. : Sophie LECOMTE

    Le groupe s'intéresse aux systèmes modèles de membranes orientés à l'interface air/eau. En particulier, il étudie la fusion membranaire, le complexe SNARE, la protéine gp41 VIH et la pénétration du virus ainsi que les complexes cyclodextrines/cholestérol.
    Le groupe
développe aussi de nombreuses méthodes optiques et des spectroscopies de vibration : IR, PMIRRAS, ATR, VCD, Raman, UV-vis, Brewster. Il maîtrise aussi les techniques du type Langmuir Blodgett.

Equipe :

Audrey AGOPIAN Isabel ALVES Marine BLANCHET
Daniel BLAUDEZ Vladimir BOBROFF Sabine CASTANO
Hsiang-Hsin CHEN Etienne HARTE Sarah HENRY
Marie-Lise JOBIN Cyril PETIBOIS Marie QUETIN

Publications :

1 - Pendem, N.; Douat-Casassus, C.; Claudon, P.; Laguerre, M.; Castano, S.; Desbat, B.; Cavagnat, D.; Ennifar, E.; Kauffmann, B.; Guichard, G.
Controlling Helix Formation in the alpha-Peptide Superfamilly: heterogeneous Foldamers with Urea/Amide and Urea/Carbamate Backbones.
Angewandte Chemie International Edition, 2013, , .
2 - Cestelli-Guidi, M.; Yao, S. D.; Sali, D.; Castano, S.; Marcelli, A.; Petibois, C.
Experimental ATR device for real-time FTIR imaging of living cells using brilliant synchrotron radiation sources.
Biotechnology Advances, 2012, in press, .
3 - Chien, C. C.; Chen, H. H.; Lai, S. F.; Wu, K. C.; Cai, X. Q.; Hwu, Y. K.; Petibois, C.; Chu, Y.; Margaritondo, G.
Gold nanoparticles as high-resolution X-ray imaging contrast agents for the analysis of tumor-related micro-vasculature.
Journal of Nanobiotechnology, 2012, 10, 12.
-Abstract-
4 - Chien, C. C.; Kempson, I. M.; Hwu, Y.; Chen, N.; Lee, T.; Petibois, C.; Tsai, K.; Liu, M.; Chang, K.; Yang, C.; Margaritondo, G.
High resolution synchrotron radiological profiling of tumour microangiogenesis.
J. Biotechnol. Adv., 2012, , .
5 - Ciaccafava, A.; de Poulpiquet, A.; Robert, S.; Gadiou, R.; Giudici-Orticoni, M. T.; Lecomte, S.; Lojou, E.
A friendly detergent for H2 oxydation by Aquifex aeolicus membrane bound hydrogenase immobilized on graphite and SAM-modified gold electrodes.
Electrochimica Acta, 2012, in press, .
6 - Ciaccafava, A.; Infossi, P.; Ilbert, M.; Guiral, M.; Lecomte, S.; Giudici-Orticoni, M. T.; Lojou, E.
Electrochemistry, AFM, and PM-IRRA Spectroscopy of Immobilized Hydrogenase: Role of a Hydrophobic Helix in Enzyme Orientation for Efficient H-2 Oxidation.
Angewandte Chemie-International Edition, 2012, 51, 953-956.
7 - Delhaye, C.; Bruneel, J. L.; Talaga, D.; Guirardel, M.; Lecomte, S.; Servant, L.
Tailoring Surface-Enhanced Raman Scattering Effect Using Microfluidics.
Journal of Physical Chemistry C, 2012, 116, 5327-5332.
-Abstract-
8 - Desbat, B.; Castano, S.
Brewster angle microscopy and imaging ellipsometry.
Encyclopedia of Biophysics, 2012, , .
9 - Dufourc, E.; Buchoux, S.; Toupe, J.; Sani, M.-A.; Jean-Francois, F.; Khemtemourian, L.; Grelard, A.; Loudet-Courreges, C.; Laguerre, M.; Elezgaray, J.; Desbat, B.; Odaert, B.
Membranes interacting peptides: From killers to helpers.
Curr. Prot. Pept. Sci., 2012, 13, 620-631.
10 - Lai, S.; Chien, C. C.; Chen, W.; Chen, H.; Chen, Y.; Wang, C.; Chen, C.; Liang, K.; Petibois, C.; Tan, H.; Tok, E.; Margaritondo, G.
Very small photoluminescent gold nanoparticles for multimodality biomedical imaging.
Biotechnol. Adv., 2012, , .
11 - Lecorche, P.; Walrant, A.; Burlina, F.; Dutot, L.; Sagan, S.; Mallet, J. M.; Desbat, B.; Chassaing, G.; Alves, I. D.; Lavielle, S.
Cellular uptake and biophysical properties of galactose and/or tryptophan containing cell-penetrating peptides.
Biochimica Et Biophysica Acta-Biomembranes, 2012, 1818, 448-457.
-Abstract-
12 - Marcelli, A.; Cricenti, A.; Kwiatek, W.; Petibois, C.
Biological applications of synchrotron radiation infrared spectromicroscopy.
Biotechnol. Adv., 2012, , .
13 - Noreen, R.; Moenner, M.; Hwu, Y.; Petibois, C.
FTIR spectro-imaging of collagens for characterizatiob and grading of gliomas.
Biotechnol. Adv., 2012, , .
14 - Petibois, C.
Morpho-spectral imaging for human disease progression investigations.
Analytical and Bioanalytical Chemistry, 2012, accepted, .
15 - Sebai, S. C.; Milioni, D.; Walrant, A.; Alves, I. D.; Sagan, S.; Huin, C.; Auvray, L.; Massotte, D.; Cribier, S.; Tribet, C.
Photocontrol of the Translocation of Molecules, Peptides, and Quantum Dots through Cell and Lipid Membranes Doped with Azobenzene Copolymers.
Angewandte Chemie-International Edition, 2012, 51, 2132-2136.
16 - Ta, H. P.; Berthelot, K.; Coulary-Salin, B.; Castano, S.; Desbat, B.; Bonnafous, P.; Lambert, O.; Alves, I.; Cullin, C.; Lecomte, S.
A yeast toxic mutant of HET-s amyloids disrupts membrane integrity.
Biochem. Biophys. Acta, 2012, 1818, 2325-2334.
17 - Taib, N.; Ahissan, A.; Moreau, L.; Camplo, M.; Houmadi, S.; Desbat, B.; Laguerre, M.; Grinstaff, M.; Bestel, I.; Barthelemy, P.
Supramolecular systems formation directed by via nucleoside-lipid recognition.
J. of Colloid and Interface Science, 2012, 337, 122-130.
18 - Taib, N.; Aime, A.; Houmadi, S.; Castano, S.; Barthelemy, P.; Laguerre, M.; Bestel, I.
Chemical details on nucleolipid supramolecular architecture: molecular modelling and physicochemical studies.
Langmuir, 2012, 28, 7452-7460.
19 - Walrant, A.; Bechara, C.; Alves, I. D.; Sagan, S.
Molecular partners for interaction and cell internalization of cell-penetrating peptides: how identical are they?.
Nanomedicine, 2012, 7, 133-143.
-Abstract-
20 - Walrant, A.; Vogel, A.; Correia, I.; Lequin, O.; Olausson, B. E.; Desbat, B.; Sagan, S.; Alves, I.
Membrane interactions of two arginine-rich peptides with different cell internalization capacities.
Biochem. Biophys. Acta, 2012, 1818, 1755-1763.
21 - Alves, I. D.; Bechara, C.; Walrant, A.; Zaltsman, Y.; Jiao, C. Y.; Sagan, S.
Relationships between Membrane Binding, Affinity and Cell Internalization Efficacy of a Cell-Penetrating Peptide: Penetratin as a Case Study.
Plos One, 2011, 6, .
-Abstract-
22 - Alves, I. D.; Staneva, G.; Tessier, C.; Salgado, G. F.; Nuss, P.
The interaction of antipsychotic drugs with lipids and subsequent lipid reorganization investigated using biophysical methods. .
BBA Biom., 2011, in press, .
23 - Banc, A.; Bauduin, P.; Desbat, B.; Ly, I.; Diat, O.
Ion Extraction Mechanism Studied in a Lyotropic Lamellar Phase.
Journal of Physical Chemistry B, 2011, 115, 1376-1384.
-Abstract-
24 - Belbachir, K.; Lecomte, S.; Ta, H. P.; Petibois, C.; Desbat, B.
Determination of the molecular group orientation in fibers by new simple methods based on polarized ATR-FTIR spectroscopy.
Analytical and Bioanalytical Chemistry, 2011, 401, 3263-3268.
25 - Belbachir, K.; Lecomte, S.; Ta, H. P.; Petibois, C.; Desbat, B.
Orientation of molecular groups of fibers in nonoriented samples determined by polarized ATR-FTIR spectroscopy.
Analytical and Bioanalytical Chemistry, 2011, 401, 3263-3268.
-Abstract-
26 - Berthelot, K.; Ta, H. P.; Gean, J.; Lecomte, S.; Cullin, C.
Antiparallel structural folding os signature of toxic amyloid.
J. Mol. Biol., 2011, 412, 137-152.
27 - Berthelot, K.; Ta, H. P.; Gean, J.; Lecomte, S.; Cullin, C.
In Vivo and In Vitro Analyses of Toxic Mutants of HET-s: FTIR Antiparallel Signature Correlates with Amyloid Toxicity.
Journal of Molecular Biology, 2011, 412, 137-152.
-Abstract-
28 - Blaudez, D.; Castano, S.; Desbat, B.
PM-IRRAS at liquid interfaces.
Biointerface characterization by IR spectroscopy, 2011, , 27-56.
29 - Cavagnat, D.; Claudon, P.; Fischer, L.; Guichard, G.; Desbat, B.
Experimental and Theoretical Study of the Vibrational Spectra of Oligoureas: Helical versus ?-Sheet-Type Secondary Structures.
J Phys Chem B. , 2011, in press, .
30 - Chen, H. H.; Chien, C. C.; Petibois, C.; Wang, C. L.; Chu, Y. S.; Lai, S. F.; Hua, T. E.; Chen, Y. Y.; Cai, X. Q.; Kempson, I. M.; Hwu, Y.; Margaritondo, G.
Quantitative analysis of nanoparticle internalization in mammalian cells by high resolution X-ray microscopy.
Journal of Nanobiotechnology, 2011, 9, 15.
-Abstract-
31 - Fezoua-Boubegtiten, Z.; Desbat, B.; Brisson, A.; Gounou, C.; Laguerre, M.; Lecomte, S.
Effect of Mg2+ versus Ca2+ on the behavior of Annexin A5 in a membrane-bound state.
European Biophysics Journal with Biophysics Letters, 2011, 40, 641-649.
-Abstract-
32 - Noreen, R.; Chien, C. C.; Delugin, M.; Yao, S. D.; Pineau, R.; Hwu, Y.; Moenner, M.; Petibois, C.
Detection of collagens in brain tumors based on FTIR imaging and chemometrics.
Analytical and Bioanalytical Chemistry, 2011, 401, 845-852.
-Abstract-
33 - Noreen, R.; Pineau, R.; Chien, C. C.; Cestelli-Guidi, M.; Hwu, Y.; Marcelli, A.; Moenner, M.; Petibois, C.
Functional histology of glioma vasculature by FTIR imaging.
Analytical and Bioanalytical Chemistry, 2011, 401, 795-801.
-Abstract-
34 - Petibois, C.
The emergence of multimodal imaging methods for real-time nanoscopy.
Analytical and Bioanalytical Chemistry, 2011, 401, 783-784.
35 - Prado, E.; Dauget, N.; Plumet, S.; Servant, L.; Lecomte, S.
Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy.
Chemical Communications, 2011, 47, 7425-7427.
36 - Prado, E.; Daugey, N.; Plumet, S.; Servant, L.; Lecomte, S.
Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy.
Chemical Communications, 2011, 47, 7425-7427.
-Abstract-
37 - Ta, H. P.; Berthelot, K.; Coulary-Salin, B.; Desbat, B.; Gean, J.; Servant, L.; Cullin, C.; Lecomte, S.
Comparative Studies of Nontoxic and Toxic Amyloids Interacting with Membrane Models at the Air-Water Interface.
Langmuir, 2011, 27, 4797-4807.
-Abstract-
38 - Walrant, A.; Correia, I.; Jiao, C. Y.; Lequin, O.; Bent, E. H.; Goasdoue, N.; Lacombe, C.; Chassaing, G.; Sagan, S.; Alves, I. D.
Different membrane behaviour and cellular uptake of three basic arginine-rich peptides.
Biochimica Et Biophysica Acta-Biomembranes, 2011, 1808, 382-393.
-Abstract-
39 - Alves, I. D.; Jiao, C. Y.; Aubry, S.; Aussedat, B.; Burlina, F.; Chassaing, G.; Sagan, S.
Cell biology meets biophysics to unveil the different mechanisms of penetratin internalization in cells.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 2231-2239.
-Abstract-
40 - Alves, I.; Rodriguez, N.; Cribier, S.; Sagan, S.
Membrane cross over by cell-penetrating peptides:kinetics and mechanisms:from model to cell membrane perturbation by permeant peptides.
Intracellular drug delivery, 2010, in press, .
41 - Berthelot, K.; Lecomte, S.; Gean, J.; Immel, F.; Cullin, C.
A Yeast Toxic Mutant of HET-s((218-289)) Prion Displays Alternative Intermediates of Amyloidogenesis.
Biophysical Journal, 2010, 99, 1239-1246.
-Abstract-
42 - Chien, C. C.; Wang, C. H.; Wang, C. L.; Li, E. R.; Lee, K. H.; Hwu, Y.; Lin, C. Y.; Chang, S. J.; Yang, C. S.; Petibois, C.; Margaritondo, G.
Synchrotron microangiography studies of angiogenesis in mice with microemulsions and gold nanoparticles.
Analytical and Bioanalytical Chemistry, 2010, 397, 2109-2116.
-Abstract-
43 - de Lima, V. R.; Caro, M. S. B.; Munford, M. L.; Desbat, B.; Dufourc, E.; Pasa, A. A.; Creczynski-Pasa, T. B.
Influence of melatonin on the order of phosphatidylcholine-based membranes.
Journal of Pineal Research, 2010, 49, 169-175.
-Abstract-
44 - Desgorces, F. D.; Taftlet, M.; Nassil, H.; Hagstrom, L.; Petibois, C.
Assessment or nutrition-related risks in athletes perspective, a study in rowers.
Fundamental & Clinical Pharmacology, 2010, 24, 183.
45 - Eid, M.; Rippa, S.; Castano, S.; Desbat, B.; Chopineau, J.; Rossi, C.; Beven, L.
Exploring the membrane mechanism of the bioactive peptaibol ampullosporin A using lipid monolayers and supported biomimetic membranes.
Journal of Biophysics, 2010, doi:10.1155/2010/179641, .
46 - Fezoua-Boubegtiten, Z.; Desbat, B.; Brisson, A.; Lecomte, S.
Determination of molecular groups involved in the interaction of annexin A5 with lipid membrane models at the air-water interface.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 1204-1211.
-Abstract-
47 - Galloux, M.; Libersou, S.; Alves, I. D.; Marquant, R.; Salgado, G. F.; Rezaei, H.; Lepault, J.; Delmas, B.; Bouaziz, S.; Morellet, N.
NMR Structure of a Viral Peptide Inserted in Artificial Membranes A VIEW ON THE EARLY STEPS OF THE BIRNAVIRUS ENTRY PROCESS.
Journal of Biological Chemistry, 2010, 285, 19409-19421.
-Abstract-
48 - Hruby, V. J.; Alves, I.; Cowell, S.; Salamon, Z.; Tollin, G.
Use of plasmon waveguide resonance (PWR) spectroscopy for examining binding, signaling and lipid domain partitioning of membrane proteins.
Life Sciences, 2010, 86, 569-574.
-Abstract-
49 - Jiao, C. Y.; Alves, I. D.; Point, V.; Lavielle, S.; Sagan, S.; Chassaing, G.
Comparing Lipid Photo-Cross-linking Efficacy of Penetratin Analogues Bearing Three Different Photoprobes: Dithienyl Ketone, Benzophenone, and Trifluoromethylaryldiazirine.
Bioconjugate Chemistry, 2010, 21, 352-359.
-Abstract-
50 - Kunsagi-Mate, S.; Lecomte, S.; Ortmann, E.; Kunsagi-Mate, E.; Desbat, B.
The environment controlled effect of thiacalix[4]arene on the transition thermodynamics and kinetics of bovine serum albumin.
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2010, 66, 147-151.
-Abstract-
51 - Maniti, O.; Alves, I.; Trugnan, G.; Ayala-Sanmartin, J.
Distinct Behaviour of the Homeodomain Derived Cell Penetrating Peptide Penetratin in Interaction with Different Phospholipids.
Plos One, 2010, 5, .
-Abstract-
52 - Petibois, C.
Imaging methods for elemental, chemical, molecular, and morphological analyses of single cells.
Analytical and Bioanalytical Chemistry, 2010, 397, 2051-2065.
-Abstract-
53 - Petibois, C.
Imaging techniques with synchrotron radiation.
Analytical and Bioanalytical Chemistry, 2010, 397, 2031-2032.
54 - Petibois, C.; Cestelli-Guidi, M.; Piccinini, M.; Moenner, M.; Marcelli, A.
Synchrotron radiation FTIR imaging in minutes: a first step towards real-time cell imaging.
Analytical and Bioanalytical Chemistry, 2010, 397, 2123-2129.
-Abstract-
55 - Petibois, C.; Desbat, B.
Clinical application of FTIR imaging: new reasons for hope.
Trends in Biotechnology, 2010, 28, 495-500.
-Abstract-
56 - Petibois, C.; Piccinini, M.; Guidi, M. C.; Marcelli, A.
Facing the challenge of biosample imaging by FTIR with a synchrotron radiation source.
Journal of Synchrotron Radiation, 2010, 17, 1-11.
-Abstract-
57 - Vie, V.; Legardinier, S.; Chieze, L.; Le Bihan, O.; Qin, Y.; Sarkis, J.; Hubert, J. F.; Renault, A.; Desbat, B.; Le Rumeur, E.
Specific anchoring modes of two distinct dystrophin rod sub-domains interacting in phospholipid Langmuir films studied by atomic force microscopy and PM-IRRAS.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 1503-1511.
-Abstract-
58 - Yao, S. D.; DellaVentura, G.; Petibois, C.
Analytical characterization of cell-asbestos fiber interactions in lung pathogenesis.
Analytical and Bioanalytical Chemistry, 2010, 397, 2079-2089.
-Abstract-
59 - Yassine, W.; Milochau, A.; Buchoux, S.; Lang, J.; Desbat, B.; Oda, R.
Effect of monolayer lipid charges on the structure and orientation of protein VAMP1 at the air-water interface.
Biochimica Et Biophysica Acta-Biomembranes, 2010, 1798, 928-937.
-Abstract-
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