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Biomedical Research: Basic and Translational (BioFAP)

The team’s research activities focus on processes involving cellular membranes, particularly those related to antibiotic resistance, membrane repair, extracellular vesicle formation, and cellular signaling in irritable bowel syndromes and glucose homeostasis. The scientific projects developed within the team aim to study and understand these processes under physiological and pathophysiological conditions (bacterial infections, cancer, diabetes, muscular dystrophies, etc.).

The team has the equipment needed to carry out a wide range of analyses using techniques in biochemistry, molecular biology, protein expression and purification—mainly applied to structural biology (Cryo-EM, X-ray crystallography)—as well as cell biology, molecular and cellular fluorescence imaging and electron microscopy, microfluidics, flow cytometry, physiology and electrophysiology, along with in vitro, in vivo, and transgenic model systems. It consists of three research groups, each led by a group leader.

Anthony BOUTER

Olivier LAMBERT

Matthieu RAOUX

Membrane Complex Architecture and Cellular Processes
Membrane Complex Architecture and Cellular Processes
Cell Biology and Biosensors
Cellular Biology and Biosensors
Membrane repair and extracellular vesicles
Membrane repair and extracellular vesicles

The team is composed of three research groups.

Architecture of Membrane Complexes and Cellular Processes

  • Olivier Lambert

Structure and function of membrane proteins: Bacterial multidrug efflux pumps and antibiotic resistance. Caveolae and cancer. Immunomodulators in vaccine strategies.

Expertise:

  • Efflux pumps
  • Antibiotic resistance
  • Electron microscopy
  • Methodological development for the study of membrane proteins.
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Cell Biology and Biosensors

  • Matthieu Raoux

Nutrient homeostasis in health and in diabetes. Intra- and intercellular signaling in the islet micro-organ. Hormone exocytosis. Cellular and micro-organ biosensors, closed-loop systems in diabetes therapy

Expertises:

  • Cell Biology
  • Molecular Biology
  • Biosensors
  • Type 1 and Type 2 Diabetes
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Membrane repair and extracellular vesicles

  • Anthony Bouter

Defective membrane repair leads to cell death and may contribute to the development of degenerative diseases, such as muscular dystrophies. Despite genetic homogeneity, muscular dystrophy patients express a range of phenotypic variability due to the presence of secondary factors, which remain to be identified. Our project aims at addressing the interplay of annexins, macrophages and fibroadipogenic progenitors in the development of muscular dystrophies, including dysferlinopathy, caveolinopathy, Duchenne muscular dystrophy, and facio-scapulo-humeral muscular dystrophy.

Expertises:

  • Extracellular vesicles (EVs)
  • Membrane repair
  • Dystrophies musculaires
  • Muscular dystrophies
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Bibliographie

  1. Gounou C., Rouyer L., Siegfried G., Harté E., Bouvet F., d’Agata L., Darbo E. , Lefeuvre M., Derieppe M.A., Bouton L., Mélane M., Chapeau D., Martineau J., Prouzet-Mauleon V., Tan S., Souleyreau W., Saltel F., Argoul F., Khatib A.M., Brisson A.R., Iggo R., Bouter A. (2024) Inhibition of the membrane repair protein annexin-A2 prevents tumor invasion and metastasis. Cell Mol Life Sci. 81(1):7.
  2. Gounou C., Bouvet F., Liet B., Prouzet-Mauléon V., d’Agata L., Harté E., Argoul F., Siegfried G., Iggo R., Khatib A..M., Bouter A. (2023) Annexin-A5 and annexin-A6 silencing prevents metastasis of breast cancer cells in zebrafish. Biol Cell. 115 (6):e202200110.
  3. Bouvet F., Ros M., Bonedeau E., Croissant C., Frelin L., Saltel F., Moreau V., Bouter A. (2020) Defective membrane repair machinery impairs survival of invasive cancer cells. Scientific Reports, 10, 1-16.

Bibliographie

  1. Ducat A., Couderc B., Bouter A., Biquard L., Aouache R., Passet B., Doridot L., Cohen M.B., Ribaux P., Apicella C., Gaillard I., Palfray S., Chen Y., Vargas A., Julé A., Frelin L., Cocquet J., Ruano C., Jacques S., Busato F., Tost J., Méhats C., Laissue P., Vilotte J.L., Miralles F., Vaiman D. (2020) Molecular mechanisms of trophoblast dysfunction mediated by imbalance between STOX1 isoforms. iSciences. 23 (5):101086. doi: 10.1016/j.isci.2020.101086.
  2. Bouter A., Carmeille R., Gounou C., Bouvet F., Degrelle S.A., Evain-Brion D., Brisson A.R. (2015) Review: Annexin-A5 and cell membrane repair. Placenta, 36 (1), S43-S49.
  3. Carmeille R., Degrelle S.A., Plawinski L., Bouvet F., Gounou C., Evain-Brion D., Brisson A.R., Bouter A. (2015) Annexin-A5 promotes membrane resealing in human trophoblasts. Biochim. Biophys. Acta., 1853, 2033-2044.
  4. Bouter A., Gounou C., Bérat R., Tan S. Gallois B., Granier T., Langlois d’Estaintot B., Pöschl E., Brachvogel B. and Brisson A.R. (2011) Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nat. Commun. 2:270 doi: 10.1038/ncomms1270.

Bibliographie

  1. d’Agata L., Rassinoux P., Gounou C., Bouvet F., Bouragba D., Mamchaoui K. and Bouter A. (2024) A novel assay reveals the early setting-up of membrane repair machinery in human skeletal muscle cells. J Cell Biochem Sept 30:e30662. doi: 10.1002/jcb.30662.
  2. Croissant C., Gounou C., Bouvet F., Tan S. and Bouter A. (2022) Trafficking of Annexins during Membrane Repair in Human Skeletal Muscle Cells. Membranes, 12(2), 153.
  3. Croissant C., Carmeille R., Brevart C. and Bouter A. (2021) Annexins and membrane repair dysfunctions in muscular dystrophies. Int. J. Mol. Sci., 22, 5276.
  4. Croissant C., Gounou C., Bouvet F., Tan S. and Bouter A. (2020) Annexin-A6 in membrane repair of human skeletal muscle cell: a role in the cap subdomain. Cells, 9, 1742. doi:10.3390/cells9071742.
  5. Croissant C., Bouvet F., Tan S. and Bouter A. (2018) Imaging membrane repair in single cells using correlative light and electron microscopy. Curr Protoc Cell Biol, e55. doi: 10.1002/cpcb.55.
  6. Carmeille R., Croissant C., Bouvet F. and Bouter A. (2017) Membrane repair assay for human skeletal muscle cells. Methods Mol Biol., 1668, 195-207.
  7. Carmeille R., Bouvet F., Tan S., Croissant C., Gounou C., Mamchaoui K., Mouly V., Brisson A.R., Bouter A. (2016) Membrane repair of human skeletal muscle cells requires Annexin-A5. Biochim. Biophys. Acta., 1863, 2267-2279.
  8. Bouter A., Gounou C., Bérat R., Tan S. Gallois B., Granier T., Langlois d’Estaintot B., Pöschl E., Brachvogel B. and Brisson A.R. (2011) Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nat. Commun. 2:270 doi: 10.1038/ncomms1270.

MSB

Biophysique multi-échelle

5 groupes de recherche

NMFAB

Applications de biomolécules pour le médical, la cosmétique, la nutraceutique, et l'agroalimentaire.

3 groupes de recherche

CBSC

Biologie chimique et Chimie supramoléculaire

5 groupes de recherche

Nos enseignants-chercheurs participe à la formation des élèves ingénieurs de Bordeaux INP (ENSMAC, ENSTBB…) et de l’université de Bordeaux.