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Cell Biology and Biosensors

Matthieu RAOUX (Pr Université de Bordeaux)

We work on transdisciplinary projects in diabetology, at both fundamental and applied levels. We investigate in particular the function of fascinating and vital micro-organs playing a central role in the regulation of blood glucose and diabetes: the pancreatic islets.

 

We mainly use approaches ranging from molecular and cellular biology, biochemistry, electrophysiology to in vivo studies. We have developed the use of sensor arrays with remarkable temporal resolution to monitor the cellular and multicellular electrical activities of islets, combined with real-time processing microelectronic circuits designed by collaborating microelectronics experts.

BioFAP Team

Cell Biology and Biosensors Group

Mathematical Modeling of Pancreatic Islets for Multi-Organ Simulation

Our data, notably from murine and human islets lacking specific cell types, can be used to construct multicellular mathematical models of islets, which are then integrated by the physicists into a multi-organ human simulator, in order to better understand the dynamic interplay between cell types within the islet cell network (ANR PRCI FUN-NET).

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Development of Real-Time Sensors for the Analysis of Ionic Species

We are also developing, through collaboration with polymer chemistry and electrochemistry experts, new real-time sensors specific to precise ion species (notably zinc, which is co-released with insulin) or to different enantiomers of amino acids (ANR PRC CHIRA-SENSEO).

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Applications of Electrophysiological Sensors in Diabetology

Our electrophysiological sensors have found several applications in diabetology, such as the functional quality control of islets before transplantation or the functional analysis of human genetic variants linked to diabetes risk in human islets derived from induced pluripotent stem cells (SFD funding).

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Microfluidic Systems 'Islets-on-a-Chip' and Artificial Pancreas

We have also developed a microfluidic islets-on-chip system to characterize the physiological responses of human islets during protocols mimicking nutrient variations as induced by meals. Based on these on-chip data, our collaborators in control theory generate islet-inspired algorithms to control commercial insulin pumps in real time in an artificial pancreas for patients with diabetes (ANR PRC MIMICbio).

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Multi-Organ Chips for Personalized Therapies

Finally, we are expanding our islets-on-chip approach to develop multi-organ and multi-sensor chips, notably in collaboration with an industrial partner, NETRI, to mimic on-chip the endocrine loop controlling blood glucose through the activity of four main organs: islets, liver, skeletal muscles and adipose tissue. Our ultimate goal aims for patient-twin-on-chip models and personalized therapies (ANR PRCE DIAMOCHIP, and RIE/University of Bordeaux).

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Matthieu
RAOUX

Professor
University of Bordeaux

Jochen
LANG

Professor
University of Bordeaux

Pier
SCOTTI

Associate Professor University of Bordeaux

Julien
GAITAN

Assistant Engineer
University of Bordeaux

Alexandra
MILOCHAU

Engineer
University of Bordeaux

Anouk
PELLERIN

Research Engineer
CNRS

Maëva
TRICHET

Research Engineer
CNRS

Valentin
SAUNIER

Postdoctoral Researcher
CNRS

Dorian
CHAPEAU

PhD Student
CNRS

Romane
LARTIGUE

PhD Student
UB

Rémi
VERGUIN

PhD Student
CNRS
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Bibliographie

  1. Gounou C., Rouyer L., Siegfried G., Harté E., Bouvet F., d’Agata L., Darbo E. , Lefeuvre M., Derieppe M.A., Bouton L., Mélane M., Chapeau D., Martineau J., Prouzet-Mauleon V., Tan S., Souleyreau W., Saltel F., Argoul F., Khatib A.M., Brisson A.R., Iggo R., Bouter A. (2024) Inhibition of the membrane repair protein annexin-A2 prevents tumor invasion and metastasis. Cell Mol Life Sci. 81(1):7.
  2. Gounou C., Bouvet F., Liet B., Prouzet-Mauléon V., d’Agata L., Harté E., Argoul F., Siegfried G., Iggo R., Khatib A..M., Bouter A. (2023) Annexin-A5 and annexin-A6 silencing prevents metastasis of breast cancer cells in zebrafish. Biol Cell. 115 (6):e202200110.
  3. Bouvet F., Ros M., Bonedeau E., Croissant C., Frelin L., Saltel F., Moreau V., Bouter A. (2020) Defective membrane repair machinery impairs survival of invasive cancer cells. Scientific Reports, 10, 1-16.

Bibliographie

  1. Ducat A., Couderc B., Bouter A., Biquard L., Aouache R., Passet B., Doridot L., Cohen M.B., Ribaux P., Apicella C., Gaillard I., Palfray S., Chen Y., Vargas A., Julé A., Frelin L., Cocquet J., Ruano C., Jacques S., Busato F., Tost J., Méhats C., Laissue P., Vilotte J.L., Miralles F., Vaiman D. (2020) Molecular mechanisms of trophoblast dysfunction mediated by imbalance between STOX1 isoforms. iSciences. 23 (5):101086. doi: 10.1016/j.isci.2020.101086.
  2. Bouter A., Carmeille R., Gounou C., Bouvet F., Degrelle S.A., Evain-Brion D., Brisson A.R. (2015) Review: Annexin-A5 and cell membrane repair. Placenta, 36 (1), S43-S49.
  3. Carmeille R., Degrelle S.A., Plawinski L., Bouvet F., Gounou C., Evain-Brion D., Brisson A.R., Bouter A. (2015) Annexin-A5 promotes membrane resealing in human trophoblasts. Biochim. Biophys. Acta., 1853, 2033-2044.
  4. Bouter A., Gounou C., Bérat R., Tan S. Gallois B., Granier T., Langlois d’Estaintot B., Pöschl E., Brachvogel B. and Brisson A.R. (2011) Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nat. Commun. 2:270 doi: 10.1038/ncomms1270.

Bibliographie

  1. d’Agata L., Rassinoux P., Gounou C., Bouvet F., Bouragba D., Mamchaoui K. and Bouter A. (2024) A novel assay reveals the early setting-up of membrane repair machinery in human skeletal muscle cells. J Cell Biochem Sept 30:e30662. doi: 10.1002/jcb.30662.
  2. Croissant C., Gounou C., Bouvet F., Tan S. and Bouter A. (2022) Trafficking of Annexins during Membrane Repair in Human Skeletal Muscle Cells. Membranes, 12(2), 153.
  3. Croissant C., Carmeille R., Brevart C. and Bouter A. (2021) Annexins and membrane repair dysfunctions in muscular dystrophies. Int. J. Mol. Sci., 22, 5276.
  4. Croissant C., Gounou C., Bouvet F., Tan S. and Bouter A. (2020) Annexin-A6 in membrane repair of human skeletal muscle cell: a role in the cap subdomain. Cells, 9, 1742. doi:10.3390/cells9071742.
  5. Croissant C., Bouvet F., Tan S. and Bouter A. (2018) Imaging membrane repair in single cells using correlative light and electron microscopy. Curr Protoc Cell Biol, e55. doi: 10.1002/cpcb.55.
  6. Carmeille R., Croissant C., Bouvet F. and Bouter A. (2017) Membrane repair assay for human skeletal muscle cells. Methods Mol Biol., 1668, 195-207.
  7. Carmeille R., Bouvet F., Tan S., Croissant C., Gounou C., Mamchaoui K., Mouly V., Brisson A.R., Bouter A. (2016) Membrane repair of human skeletal muscle cells requires Annexin-A5. Biochim. Biophys. Acta., 1863, 2267-2279.
  8. Bouter A., Gounou C., Bérat R., Tan S. Gallois B., Granier T., Langlois d’Estaintot B., Pöschl E., Brachvogel B. and Brisson A.R. (2011) Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nat. Commun. 2:270 doi: 10.1038/ncomms1270.

MSB

Biophysique multi-échelle

5 groupes de recherche

NMFAB

Applications de biomolécules pour le médical, la cosmétique, la nutraceutique, et l'agroalimentaire.

3 groupes de recherche

CBSC

Biologie chimique et Chimie supramoléculaire

5 groupes de recherche

Nos enseignants-chercheurs participe à la formation des élèves ingénieurs de Bordeaux INP (ENSMAC, ENSTBB…) et de l’université de Bordeaux.