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Structure and activity of biological macromolecules

Gilles JOUCLA

The Structure and Activities of Biological Macromolecules (SAMB) group at CBMN develops innovative approaches in bioproduction, purification, and characterization of therapeutic biomolecules, as well as in tumor mechanobiology and biotechnologies for implantation and bioproduction. The group is particularly involved in the functionalization of lipoprotein complexes (LPC) for the diagnosis and targeted treatment of atherosclerosis. In addition, its research combines bioprocesses in bioreactors and biomimetic 3D models to better understand cell–microenvironment interactions. The objective is to optimize the production of recombinant proteins while exploring new strategies to modulate tumor migration and develop innovative therapies.

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NMFAB Team

Group Structure and Activity of Biological Macromolecules

Development of production and purification processes for therapeutic biomolecules

The SAMB team on Axis 1 develops optimized processes for the production and purification of biomolecules of therapeutic interest, with a particular focus on recombinant proteins (antibodies and antibody fragments, mRNA…).

Projects in collaboration with UMR 5536/CRMSB:

“Antibody-Functionalized Lipoprotein Complexes (LPC) including ApoA1 and a repolarizing drug for the targeted therapy of atherosclerosis”
Project leader: Dr. Gisèle CLOFENT-SANCHEZ (CRMSB–UMR 5536–CNRS/UB)
Funding: ANR AAPG2022
Collaboration with the SAMB team: Dr. Gilles JOUCLA, Prof. Charlotte CABANNE, Dr. Abdelmajid NOUBHANI, Dr. Wilfrid DIERYCK, Kim BENETEAU

The LPCBioMab project aims to develop a targeted therapy against atherosclerosis by combining biomimetic lipoprotein complexes based on Apolipoprotein A1 (ApoA1) with a companion diagnostic. These complexes, enriched with native ApoA1 and functionalized with specific antibodies, target inflammatory macrophages and oxidized ApoA1 to restore lipid homeostasis and modulate plaque inflammation. The project also integrates an MRI imaging approach via iron-platinum nanoparticles for therapeutic monitoring.

“Human Antibody-enabled Cardiovascular Personalized Theranosis (ABCardionostics)”
Project leader: Dr. Gisèle CLOFENT-SANCHEZ (CRMSB–UMR 5536–CNRS/UB)
Funding: European project April 2024 – 2028
Collaboration with the SAMB team: Dr. Gilles JOUCLA, Prof. Charlotte CABANNE, Dr. Abdelmajid NOUBHANI, Dr. Wilfrid DIERYCK

The European ABCardionostics project is developing a multi-marker PET/MRI system to improve the diagnosis and personalized management of patients with vulnerable atherosclerosis. It relies on human antibodies targeting macrophages involved in plaque progression, enabling a detailed analysis of their composition and evolution. Through advanced technologies (phage display, multispectral mapping, antibody bioengineering), the project aims to introduce new imaging and immunotherapy strategies to prevent cardiovascular complications.

     → https://abcardionostics.eu/

Projects in collaboration on production processes:

  • Project “DNA / RNA vectorization with modified dextran/chitosan polymers for transfection and expression in mammalian cells”
    Project leader: Christophe SCHATZ (LCPO)
    Collaboration with the SAMB team: Dr. Gilles JOUCLA

  • Project “Search for antibiotic-active molecules in macrofungi”
    Project leader: Sébastien VILAIN (CBMN)
    Collaboration with the SAMB team: Laurent BONNEAU

  • Project “ENDOWINE”: Endolysins derived from bacteriophages in fermented foods
    Project leaders: Claire LE HÉNAFF (UMR Oenology) & Charlotte BRIVES (Sciences Po Bordeaux)
    Collaboration with the SAMB team: Dr. Agnès HOCQUELLET

  • Project “THERagnostic probe directed towards Oxidized and Mutant Apolipoprotein”
    Project leader: Laurent AZEMA: ARNA (INSERM U1212 / CNRS UMR 5320) & Laetitia DAURY-JOUCLA (O. LAMBERT team)
    Funding: AAP 2021 STS Department / Aptamers
    Collaboration with the SAMB team: Dr. Abdelmajid NOUBHANI, Dr. Wilfrid DIERYCK

  • Project “Production, purification, and characterization of NLP (Necrosis and Ethylene-inducing Peptide1-Like Proteins)”
    Project leaders: Antoine LOQUET (IECB, CBMN) & Sébastien MONGRAND (Membrane Biogenesis)
    Funding: S. MONGRAND team
    Collaboration with the SAMB team: Dr. Abdelmajid NOUBHANI

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Mechanobiology, cell–environment interactions, cell signaling, and biomimetic models

Project Mechanosensing, Coordinator: Dr. Emilie MARHUENDA

“Glioblastoma Stem Cell Migration and Immune Modulation Using Functionalized Nanofiber Scaffolds in Zebrafish”, Coordinators: Dr. Emilie MARHUENDA / Prof. Norbert BAKALARA

Academic partners: Géraldine SIEGFRIED (University of Bordeaux, Inserm…), David CORNU and Julien CAMBEDOUZOU (IEM Montpellier, CNRS…), Prof. Hugues DUFFAU / Luc BAUCHET / Jean-Phillipe HUGNOT

 

Project: “Next-Generation Microcarriers: Tunable 3D Fiber Matrices for Continuous MSC-Derived Exosome Biomanufacturing”, Coordinators: Dr. Emilie MARHUENDA / Dr. Gilles JOUCLA / Prof. Norbert BAKALARA

Academic partners: David CORNU and Julien CAMBEDOUZOU (IEM Montpellier, CNRS…)

 

Project: “Impact of Mechanosensitivity on Exosomal Communication in a Biomimetic Microenvironment”, Coordinators: Dr. Emilie MARHUENDA / Prof. Norbert BAKALARA

The stiffness of the tumor microenvironment strongly influences intercellular communication, particularly through extracellular vesicles (EVs) such as exosomes. Using a 3D co-culture model on electrospun fibers with controlled stiffness, we will analyze how tumor stem cell mechanosensitivity affects exosome production and content, and in turn, how these exosomes influence fibroblast activation and differentiation into Cancer-Associated Fibroblasts (CAF). The aim of this project is to highlight perspectives for targeting exosomal signaling as a therapeutic lever.

 

Project: “Screening of Therapeutic Molecules under Well-Defined Mechanical Conditions in a 3D Biomimetic Platform”, Coordinators: Dr. Emilie MARHUENDA / Prof. Norbert BAKALARA / Dr. Gilles JOUCLA

Characterization of innovative molecules in a 3D culture system, focusing on their antiproliferative, cytotoxic, and anti-migratory effects on invasive cancers.

  • “Effects of Bacterial Culture Supernatants from the Garonne Riverbed on Glioblastoma Growth”, Laurent BONNEAU, Prof. Norbert BAKALARA, Dr. Gilles JOUCLA
    Academic partners: Dr. Sébastien VILAIN (CBMN)

  • “Development of a Cell Culture Model to Test the Anticancer Biological Activity of Natural Phenolic Extracts”, Dr. Gilles JOUCLA, Laurent BONNEAU, Prof. Norbert BAKALARA
    Academic partners: Dr. Elise DARGELOS (CBMN)

  • “Characterization of Antiproliferative and Anti-Migratory Agents Against Invasive Cancers and Healthy Stem Cells (anti-HDAC6)”, Prof. Norbert BAKALARA, Joint project with Mexico

Learn more

Gilles
JOUCLA

Associate Professor
Bordeaux INP

Norbert
BAKALARA

Professor
Bordeaux INP

Charlotte
CABANNE

Professor
Bordeaux INP

Jean-Marc
CHEVALIER

Research Engineer
CNRS

Patricia
COSTAGLIOLI

Associate Professor
Bordeaux INP

Wilfrid
DIERYCK

Associate Professor
University of Bordeaux

Bertrand
GARBAY

Professor
Bordeaux INP

Agnès
HOCQUELLET

Associate Professor
Bordeaux INP

Emilie
MARHUENDA

Associate Professor
Bordeaux INP

Abdelmajid
NOUBAHNI

Associate Professor
Bordeaux INP

Laurent
BONNEAU

Maître de Conférences Bordeaux INP
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Bibliographie

  1. Gounou C., Rouyer L., Siegfried G., Harté E., Bouvet F., d’Agata L., Darbo E. , Lefeuvre M., Derieppe M.A., Bouton L., Mélane M., Chapeau D., Martineau J., Prouzet-Mauleon V., Tan S., Souleyreau W., Saltel F., Argoul F., Khatib A.M., Brisson A.R., Iggo R., Bouter A. (2024) Inhibition of the membrane repair protein annexin-A2 prevents tumor invasion and metastasis. Cell Mol Life Sci. 81(1):7.
  2. Gounou C., Bouvet F., Liet B., Prouzet-Mauléon V., d’Agata L., Harté E., Argoul F., Siegfried G., Iggo R., Khatib A..M., Bouter A. (2023) Annexin-A5 and annexin-A6 silencing prevents metastasis of breast cancer cells in zebrafish. Biol Cell. 115 (6):e202200110.
  3. Bouvet F., Ros M., Bonedeau E., Croissant C., Frelin L., Saltel F., Moreau V., Bouter A. (2020) Defective membrane repair machinery impairs survival of invasive cancer cells. Scientific Reports, 10, 1-16.

Bibliographie

  1. Ducat A., Couderc B., Bouter A., Biquard L., Aouache R., Passet B., Doridot L., Cohen M.B., Ribaux P., Apicella C., Gaillard I., Palfray S., Chen Y., Vargas A., Julé A., Frelin L., Cocquet J., Ruano C., Jacques S., Busato F., Tost J., Méhats C., Laissue P., Vilotte J.L., Miralles F., Vaiman D. (2020) Molecular mechanisms of trophoblast dysfunction mediated by imbalance between STOX1 isoforms. iSciences. 23 (5):101086. doi: 10.1016/j.isci.2020.101086.
  2. Bouter A., Carmeille R., Gounou C., Bouvet F., Degrelle S.A., Evain-Brion D., Brisson A.R. (2015) Review: Annexin-A5 and cell membrane repair. Placenta, 36 (1), S43-S49.
  3. Carmeille R., Degrelle S.A., Plawinski L., Bouvet F., Gounou C., Evain-Brion D., Brisson A.R., Bouter A. (2015) Annexin-A5 promotes membrane resealing in human trophoblasts. Biochim. Biophys. Acta., 1853, 2033-2044.
  4. Bouter A., Gounou C., Bérat R., Tan S. Gallois B., Granier T., Langlois d’Estaintot B., Pöschl E., Brachvogel B. and Brisson A.R. (2011) Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nat. Commun. 2:270 doi: 10.1038/ncomms1270.

Bibliographie

  1. d’Agata L., Rassinoux P., Gounou C., Bouvet F., Bouragba D., Mamchaoui K. and Bouter A. (2024) A novel assay reveals the early setting-up of membrane repair machinery in human skeletal muscle cells. J Cell Biochem Sept 30:e30662. doi: 10.1002/jcb.30662.
  2. Croissant C., Gounou C., Bouvet F., Tan S. and Bouter A. (2022) Trafficking of Annexins during Membrane Repair in Human Skeletal Muscle Cells. Membranes, 12(2), 153.
  3. Croissant C., Carmeille R., Brevart C. and Bouter A. (2021) Annexins and membrane repair dysfunctions in muscular dystrophies. Int. J. Mol. Sci., 22, 5276.
  4. Croissant C., Gounou C., Bouvet F., Tan S. and Bouter A. (2020) Annexin-A6 in membrane repair of human skeletal muscle cell: a role in the cap subdomain. Cells, 9, 1742. doi:10.3390/cells9071742.
  5. Croissant C., Bouvet F., Tan S. and Bouter A. (2018) Imaging membrane repair in single cells using correlative light and electron microscopy. Curr Protoc Cell Biol, e55. doi: 10.1002/cpcb.55.
  6. Carmeille R., Croissant C., Bouvet F. and Bouter A. (2017) Membrane repair assay for human skeletal muscle cells. Methods Mol Biol., 1668, 195-207.
  7. Carmeille R., Bouvet F., Tan S., Croissant C., Gounou C., Mamchaoui K., Mouly V., Brisson A.R., Bouter A. (2016) Membrane repair of human skeletal muscle cells requires Annexin-A5. Biochim. Biophys. Acta., 1863, 2267-2279.
  8. Bouter A., Gounou C., Bérat R., Tan S. Gallois B., Granier T., Langlois d’Estaintot B., Pöschl E., Brachvogel B. and Brisson A.R. (2011) Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nat. Commun. 2:270 doi: 10.1038/ncomms1270.

MSB

Biophysique multi-échelle

5 groupes de recherche

NMFAB

Applications de biomolécules pour le médical, la cosmétique, la nutraceutique, et l'agroalimentaire.

3 groupes de recherche

CBSC

Biologie chimique et Chimie supramoléculaire

5 groupes de recherche

Nos enseignants-chercheurs participe à la formation des élèves ingénieurs de Bordeaux INP (ENSMAC, ENSTBB…) et de l’université de Bordeaux.